Variation in the hypervariable region of cucumber mosaic virus satellite RNAs is affected by the helper virusand the initial sequence context

Peter Palukaitis, Marilyn J. Roossinck

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The D satellite RNA (sat RNA) of cucumber mosaic virus (CMV) was previously shown to contain a region of hypervariability around nucleotide 230, in wild-type populations and in cDNA clones and progeny of one such clone (pDsat4) after passage with the subgroup I strain Fny-CMV. This hypervariable region (HVR) consists of a series of consecutive A and/or U residues. We found that variability is also generated in the HVR of transcript derived from pDsat4 after passage with the subgroup II strain LS-CMV and with tomato aspermy virus (TAV). However, the progeny differ with respect to the sequence of the HVR after passage with both LS-CMV and TAV. Another D-sat RNA cDNA clone that contains a C residue in the HVR, pDsat1, was previously shown not to develop variability in the HVR upon passage with Fny-CMV. However, when the C (position 231) was changed to an A residue, variability developed by the third passage with Fny-CMV. An additional cDNA clone derived from the B1-sat RNA, pBsat5, also contains a C residue in the region analogous to the D-sat RNA HVR and did not develop variability upon passage with either Fny- or LS-CMV. Changing this C to a U residue did not result in the development of hypervariability in the progeny of transcript from this mutant. Models to explain the generation of hypervariability are discussed.

Original languageEnglish (US)
Article number95800069
Pages (from-to)765-768
Number of pages4
JournalVirology
Volume206
Issue number1
DOIs
StatePublished - Jan 10 1995

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Cucumber Mosaic Virus Satellite
Cucumovirus
Satellite RNAs
Clone Cells
Complementary DNA
Lycopersicon esculentum
Viruses
Nucleotides

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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title = "Variation in the hypervariable region of cucumber mosaic virus satellite RNAs is affected by the helper virusand the initial sequence context",
abstract = "The D satellite RNA (sat RNA) of cucumber mosaic virus (CMV) was previously shown to contain a region of hypervariability around nucleotide 230, in wild-type populations and in cDNA clones and progeny of one such clone (pDsat4) after passage with the subgroup I strain Fny-CMV. This hypervariable region (HVR) consists of a series of consecutive A and/or U residues. We found that variability is also generated in the HVR of transcript derived from pDsat4 after passage with the subgroup II strain LS-CMV and with tomato aspermy virus (TAV). However, the progeny differ with respect to the sequence of the HVR after passage with both LS-CMV and TAV. Another D-sat RNA cDNA clone that contains a C residue in the HVR, pDsat1, was previously shown not to develop variability in the HVR upon passage with Fny-CMV. However, when the C (position 231) was changed to an A residue, variability developed by the third passage with Fny-CMV. An additional cDNA clone derived from the B1-sat RNA, pBsat5, also contains a C residue in the region analogous to the D-sat RNA HVR and did not develop variability upon passage with either Fny- or LS-CMV. Changing this C to a U residue did not result in the development of hypervariability in the progeny of transcript from this mutant. Models to explain the generation of hypervariability are discussed.",
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Variation in the hypervariable region of cucumber mosaic virus satellite RNAs is affected by the helper virusand the initial sequence context. / Palukaitis, Peter; Roossinck, Marilyn J.

In: Virology, Vol. 206, No. 1, 95800069, 10.01.1995, p. 765-768.

Research output: Contribution to journalArticle

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