Variation in the ribosomal DNA internal transcribed spacer of a diverse collection of ectomycorrhizal fungi

David J. Farmer, David M. Sylvia

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Amplification of the internal transcribed spacer (ITS) of ribosomal RNA genes using PCR and subsequent RFLP analysis offers promise for improved species-level identification of ectomycorrhizal fungi. Intrageneric homogeneity and intraspecific heterogeneity of these characters are, however, frequent. A diverse group of ectomycorrhizal fungi with worldwide distribution was characterized by ITS-RFLP analysis. DNA of 64 identified and five unknown ectomycorrhizal fungi was extracted and their ITS regions were selectively amplified with ITS1-F and ITS4 ribosomal RNA gene primers. Each PCR product was digested with seven restriction enzymes. The resulting restriction fragments were compared by distance matrix analysis. Restriction enzyme analysis of amplified fragments was sufficient for distinguishing all but two closely related species. Intraspecific variation was observed in Cenococcum geophilum and Pisolithus arhizus, providing further evidence that these taxa represent species complexes.

Original languageEnglish (US)
Pages (from-to)859-865
Number of pages7
JournalMycological Research
Volume102
Issue number7
DOIs
StatePublished - Jul 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'Variation in the ribosomal DNA internal transcribed spacer of a diverse collection of ectomycorrhizal fungi'. Together they form a unique fingerprint.

Cite this