Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica)

Youmie Park, Zhenqing Zhang, Tatiana N. Laremore, Boyangzi Li, Joon Soo Sim, A. Rang Im, Mi Young Ahn, Yeong Shik Kim, Robert J. Linhardt

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide →4)-α-d-GlcNpAc (1→4)-α-l-IdoAp2S(1→, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex2-4-HexNAc2), high mannose (Hex5-9-HexNAc2), and complex (Hex3-HexNAc 2-10) types. None showed core fucosylation.

Original languageEnglish (US)
Pages (from-to)863-877
Number of pages15
JournalGlycoconjugate Journal
Volume25
Issue number9
DOIs
StatePublished - Dec 1 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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