VdSNF1, the sucrose nonfermenting protein kinase gene of verticillium dahliae, is required for virulence and expression of genes involved in cell-wall degradation

Aliki K. Tzima, Epaminondas J. Paplomatas, Payungsak Rauyaree, Manuel D. Ospina-Giraldo, Seogchan Kang

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Verticillium dahliae is a soilborne fungus causing vascular wilt in a diverse array of plant species. Its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall-degrading enzymes (CWDE). The sucrose nonfermenting 1 gene (VdSNF1), which regulates catabolic repression, was disrupted in V. dahliae tomato race 1. Expression of CWDE in the resulting mutants was not induced in inductive medium and in simulated xylem fluid medium. Growth of the mutants was significantly reduced when grown with pectin or galactose as a carbon source whereas, with glucose, sucrose, and xylose, they grew similarly to wild-type and ectopic transformants. The mutants were severely impaired in virulence on tomato and eggplant (final disease severity reduced by an average of 87%). Microscopic observation of the infection behavior of a green fluorescent protein (gfp)-labeled VdSNF1 mutant (70ΔSF-gfp1) showed that it was defective in initial colonization of roots. Cross sections of tomato stem at the cotyledonary level showed that 70ΔSF-gfp1 colonized xylem vessels considerably less than the wild-type strain. The wildtype strain heavily colonized xylem vessels and adjacent parenchyma cells. Quantification of fungal biomass in plant tissues further confirmed reduced colonization of roots, stems, and cotyledons by 70ΔSF-gfp1 relative to that by the wild-type strain.

Original languageEnglish (US)
Pages (from-to)129-142
Number of pages14
JournalMolecular Plant-Microbe Interactions
Volume24
Issue number1
DOIs
StatePublished - Jan 2011

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Verticillium
Xylem
Verticillium dahliae
Lycopersicon esculentum
protein kinases
Cell Wall
Protein Kinases
Virulence
Sucrose
virulence
cell walls
sucrose
Gene Expression
gene expression
mutants
degradation
xylem vessels
tomatoes
Solanum melongena
Genes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

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title = "VdSNF1, the sucrose nonfermenting protein kinase gene of verticillium dahliae, is required for virulence and expression of genes involved in cell-wall degradation",
abstract = "Verticillium dahliae is a soilborne fungus causing vascular wilt in a diverse array of plant species. Its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall-degrading enzymes (CWDE). The sucrose nonfermenting 1 gene (VdSNF1), which regulates catabolic repression, was disrupted in V. dahliae tomato race 1. Expression of CWDE in the resulting mutants was not induced in inductive medium and in simulated xylem fluid medium. Growth of the mutants was significantly reduced when grown with pectin or galactose as a carbon source whereas, with glucose, sucrose, and xylose, they grew similarly to wild-type and ectopic transformants. The mutants were severely impaired in virulence on tomato and eggplant (final disease severity reduced by an average of 87{\%}). Microscopic observation of the infection behavior of a green fluorescent protein (gfp)-labeled VdSNF1 mutant (70ΔSF-gfp1) showed that it was defective in initial colonization of roots. Cross sections of tomato stem at the cotyledonary level showed that 70ΔSF-gfp1 colonized xylem vessels considerably less than the wild-type strain. The wildtype strain heavily colonized xylem vessels and adjacent parenchyma cells. Quantification of fungal biomass in plant tissues further confirmed reduced colonization of roots, stems, and cotyledons by 70ΔSF-gfp1 relative to that by the wild-type strain.",
author = "Tzima, {Aliki K.} and Paplomatas, {Epaminondas J.} and Payungsak Rauyaree and Ospina-Giraldo, {Manuel D.} and Seogchan Kang",
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VdSNF1, the sucrose nonfermenting protein kinase gene of verticillium dahliae, is required for virulence and expression of genes involved in cell-wall degradation. / Tzima, Aliki K.; Paplomatas, Epaminondas J.; Rauyaree, Payungsak; Ospina-Giraldo, Manuel D.; Kang, Seogchan.

In: Molecular Plant-Microbe Interactions, Vol. 24, No. 1, 01.2011, p. 129-142.

Research output: Contribution to journalArticle

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T1 - VdSNF1, the sucrose nonfermenting protein kinase gene of verticillium dahliae, is required for virulence and expression of genes involved in cell-wall degradation

AU - Tzima, Aliki K.

AU - Paplomatas, Epaminondas J.

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AU - Ospina-Giraldo, Manuel D.

AU - Kang, Seogchan

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AB - Verticillium dahliae is a soilborne fungus causing vascular wilt in a diverse array of plant species. Its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall-degrading enzymes (CWDE). The sucrose nonfermenting 1 gene (VdSNF1), which regulates catabolic repression, was disrupted in V. dahliae tomato race 1. Expression of CWDE in the resulting mutants was not induced in inductive medium and in simulated xylem fluid medium. Growth of the mutants was significantly reduced when grown with pectin or galactose as a carbon source whereas, with glucose, sucrose, and xylose, they grew similarly to wild-type and ectopic transformants. The mutants were severely impaired in virulence on tomato and eggplant (final disease severity reduced by an average of 87%). Microscopic observation of the infection behavior of a green fluorescent protein (gfp)-labeled VdSNF1 mutant (70ΔSF-gfp1) showed that it was defective in initial colonization of roots. Cross sections of tomato stem at the cotyledonary level showed that 70ΔSF-gfp1 colonized xylem vessels considerably less than the wild-type strain. The wildtype strain heavily colonized xylem vessels and adjacent parenchyma cells. Quantification of fungal biomass in plant tissues further confirmed reduced colonization of roots, stems, and cotyledons by 70ΔSF-gfp1 relative to that by the wild-type strain.

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