Vector specificity of Barley Yellow Dwarf Virus (BYDV) transmission

Identification of potential cellular receptors binding BYDV-MAV in the aphid, Sitobion avenae

Chaoyang Li, Diana Lynn Cox-foster, Stewart M. Gray, Frederick Gildow

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Two proteins (SaM35 and SaM50) isolated from head tissues of the aphid vector, Sitobion avenae, were identified as potential receptors for barley yellow dwarf virus MAV isolate (Luteoviridae) based on MAV virus overlay assays and immunoblots of urea SDS 2-D gels. An anti-idiotypic antibody (MAV4 anti-ID) that mimics an epitope on MAV virions and competes with MAV in antibody binding assays also bound to SaM50 and SaM35 and to six additional proteins including a GroEL homolog No MAV-binding proteins were detected from the nonvector aphid, Rhopatosiphum maidis, although MAV4 anti-ID did react with four proteins from R. maidis. It is hypothesized that SaM35 and SaM50 may be MAV receptors involved in MAV transmission based on their high affinity for MAV and their unique association with the vector, S. avenae. The additional aphid proteins binding the MAV4 anti-ID may represent less specific virus-binding proteins facilitating transmission through different aphid tissues.

Original languageEnglish (US)
Pages (from-to)125-133
Number of pages9
JournalVirology
Volume286
Issue number1
DOIs
StatePublished - Jul 20 2001

Fingerprint

Luteovirus
Aphids
Luteoviridae
Carrier Proteins
Virus Attachment
Proteins
Protein Binding
Virion
Urea
Epitopes
Anti-Idiotypic Antibodies
Gels
Head
Viruses
Antibodies

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Li, Chaoyang ; Cox-foster, Diana Lynn ; Gray, Stewart M. ; Gildow, Frederick. / Vector specificity of Barley Yellow Dwarf Virus (BYDV) transmission : Identification of potential cellular receptors binding BYDV-MAV in the aphid, Sitobion avenae. In: Virology. 2001 ; Vol. 286, No. 1. pp. 125-133.
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abstract = "Two proteins (SaM35 and SaM50) isolated from head tissues of the aphid vector, Sitobion avenae, were identified as potential receptors for barley yellow dwarf virus MAV isolate (Luteoviridae) based on MAV virus overlay assays and immunoblots of urea SDS 2-D gels. An anti-idiotypic antibody (MAV4 anti-ID) that mimics an epitope on MAV virions and competes with MAV in antibody binding assays also bound to SaM50 and SaM35 and to six additional proteins including a GroEL homolog No MAV-binding proteins were detected from the nonvector aphid, Rhopatosiphum maidis, although MAV4 anti-ID did react with four proteins from R. maidis. It is hypothesized that SaM35 and SaM50 may be MAV receptors involved in MAV transmission based on their high affinity for MAV and their unique association with the vector, S. avenae. The additional aphid proteins binding the MAV4 anti-ID may represent less specific virus-binding proteins facilitating transmission through different aphid tissues.",
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Vector specificity of Barley Yellow Dwarf Virus (BYDV) transmission : Identification of potential cellular receptors binding BYDV-MAV in the aphid, Sitobion avenae. / Li, Chaoyang; Cox-foster, Diana Lynn; Gray, Stewart M.; Gildow, Frederick.

In: Virology, Vol. 286, No. 1, 20.07.2001, p. 125-133.

Research output: Contribution to journalArticle

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