Vesicle impact electrochemical cytometry compared to amperometric exocytosis measurements

Johan Dunevall, Soodabeh Majdi, Anna Larsson, Andrew Ewing

Research output: Contribution to journalReview articlepeer-review

19 Scopus citations

Abstract

Three new tools are discussed for understanding chemical communication between cells and primarily to delve into the content and structure of nanometer transmitter vesicles. These are amperometric measurements of exocytosis, vesicle impact electrochemical cytometry, and intracellular vesicle impact electrochemical cytometry. These are combining in the end nanoscale mass spectrometry imaging to begin determination of vesicle structure. These methods have provided solid evidence for the concept of open and closed exocytosis leading to partial release of the vesicle content during normal exocytosis. They have also been used to discover cases where the fraction of transmitter released is not changed, and other cases where the vesicle transmitter fraction released is altered, as with zinc, thought to alter cognition. Overall, the combination of these methods is showing us details of vesicular processes that would not be measureable without these micro and nano electrochemical methods.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalCurrent Opinion in Electrochemistry
Volume5
Issue number1
DOIs
StatePublished - Oct 2017

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry

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