Viability of human marrow after long-term cryopreservation

Witold Rybka, Karl Mittermeyer, Jack W. Singer, C. Dean Buckner, E. Donnall Thomas

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human bone marrow specimens cryopreserved with dimethyl sulfoxide (Me2SO) and stored in the vapor phase of liquid nitrogen for 37 to 121 months were evaluated for viability using the granulocyte—macrophage (CFU-C) and burst-forming (BFU-E) assays. Eighteen marrows were from patients with a variety of malignant diseases and 10 were from patients with chronic granulocytic leukemia (CGL). Twenty-two of the twenty-eight stored marrows showed in vitro colony growth by at least one of the assays. Four of the marrows from patients with malignant diseases and two from patients with CGL showed no growth. All marrows showing no colony growth had been stored at least 8.5 years, although colony growth was seen in marrows stored for up to 10 years.

Original languageEnglish (US)
Pages (from-to)424-428
Number of pages5
JournalCryobiology
Volume17
Issue number5
DOIs
StatePublished - Jan 1 1980

Fingerprint

Cryopreservation
cryopreservation
Bone Marrow
viability
leukemia
Assays
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Growth
assays
Liquid nitrogen
dimethyl sulfoxide
Dimethyl Sulfoxide
vapors
bone marrow
Erythroid Precursor Cells
Bone
Vapors
liquids
nitrogen
Nitrogen

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Rybka, W., Mittermeyer, K., Singer, J. W., Buckner, C. D., & Thomas, E. D. (1980). Viability of human marrow after long-term cryopreservation. Cryobiology, 17(5), 424-428. https://doi.org/10.1016/0011-2240(80)90051-6
Rybka, Witold ; Mittermeyer, Karl ; Singer, Jack W. ; Buckner, C. Dean ; Thomas, E. Donnall. / Viability of human marrow after long-term cryopreservation. In: Cryobiology. 1980 ; Vol. 17, No. 5. pp. 424-428.
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Rybka, W, Mittermeyer, K, Singer, JW, Buckner, CD & Thomas, ED 1980, 'Viability of human marrow after long-term cryopreservation', Cryobiology, vol. 17, no. 5, pp. 424-428. https://doi.org/10.1016/0011-2240(80)90051-6

Viability of human marrow after long-term cryopreservation. / Rybka, Witold; Mittermeyer, Karl; Singer, Jack W.; Buckner, C. Dean; Thomas, E. Donnall.

In: Cryobiology, Vol. 17, No. 5, 01.01.1980, p. 424-428.

Research output: Contribution to journalArticle

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Rybka W, Mittermeyer K, Singer JW, Buckner CD, Thomas ED. Viability of human marrow after long-term cryopreservation. Cryobiology. 1980 Jan 1;17(5):424-428. https://doi.org/10.1016/0011-2240(80)90051-6