Viable but non-culturable and persistence describe the same bacterial stress state

Jun Seob Kim, Nityananda Chowdhury, Ryota Yamasaki, Thomas Keith Wood

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Bacteria are often thought of as having two dormant phenotypes: the viable but non-culturable (VBNC) state and the persister state. Here we investigate the relatedness of the two stress-induced phenotypes at the single-cell level and examine cell morphology and quantify cell resuscitation. Using the classic starvation conditions to create VBNC cells, we found that the majority of the remaining Escherichia coli population are spherical, have empty cytosol and fail to resuscitate; however, some of the spherical cells resuscitate immediately (most probably those with dense cytosol). Critically, all the culturable cells in this starved population became persister cells within 14 days of starvation. We found that the persister cells initially are rod-like, have clear but limited membrane damage, can resuscitate immediately and gradually become spherical by aging. After 24 h, only rod-shaped persister cells survive, and all the spherical cells lyse. Both cell populations formed under the VBNC-inducing conditions and the persister conditions are metabolically inactive. Therefore, the bacterial population consists of dead cells and persister cells in the VBNC-inducing conditions; that is, the non-lysed particles that do not resuscitate are dead, and the dormant cells that resuscitate are persister cells. Hence, ‘VBNC’ and ‘persister’ describe the same dormant phenotype.

Original languageEnglish (US)
Pages (from-to)2038-2048
Number of pages11
JournalEnvironmental microbiology
Volume20
Issue number6
DOIs
StatePublished - Jun 1 2018

Fingerprint

phenotype
persistence
starvation
lysis
cells
relatedness
membrane
damage
bacterium
Starvation
Phenotype
cytosol
Cytosol
Population
Resuscitation
particle
Escherichia coli
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Kim, Jun Seob ; Chowdhury, Nityananda ; Yamasaki, Ryota ; Wood, Thomas Keith. / Viable but non-culturable and persistence describe the same bacterial stress state. In: Environmental microbiology. 2018 ; Vol. 20, No. 6. pp. 2038-2048.
@article{7042a4b2d9c5421ebe373605d8ca5d7f,
title = "Viable but non-culturable and persistence describe the same bacterial stress state",
abstract = "Bacteria are often thought of as having two dormant phenotypes: the viable but non-culturable (VBNC) state and the persister state. Here we investigate the relatedness of the two stress-induced phenotypes at the single-cell level and examine cell morphology and quantify cell resuscitation. Using the classic starvation conditions to create VBNC cells, we found that the majority of the remaining Escherichia coli population are spherical, have empty cytosol and fail to resuscitate; however, some of the spherical cells resuscitate immediately (most probably those with dense cytosol). Critically, all the culturable cells in this starved population became persister cells within 14 days of starvation. We found that the persister cells initially are rod-like, have clear but limited membrane damage, can resuscitate immediately and gradually become spherical by aging. After 24 h, only rod-shaped persister cells survive, and all the spherical cells lyse. Both cell populations formed under the VBNC-inducing conditions and the persister conditions are metabolically inactive. Therefore, the bacterial population consists of dead cells and persister cells in the VBNC-inducing conditions; that is, the non-lysed particles that do not resuscitate are dead, and the dormant cells that resuscitate are persister cells. Hence, ‘VBNC’ and ‘persister’ describe the same dormant phenotype.",
author = "Kim, {Jun Seob} and Nityananda Chowdhury and Ryota Yamasaki and Wood, {Thomas Keith}",
year = "2018",
month = "6",
day = "1",
doi = "10.1111/1462-2920.14075",
language = "English (US)",
volume = "20",
pages = "2038--2048",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "6",

}

Viable but non-culturable and persistence describe the same bacterial stress state. / Kim, Jun Seob; Chowdhury, Nityananda; Yamasaki, Ryota; Wood, Thomas Keith.

In: Environmental microbiology, Vol. 20, No. 6, 01.06.2018, p. 2038-2048.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Viable but non-culturable and persistence describe the same bacterial stress state

AU - Kim, Jun Seob

AU - Chowdhury, Nityananda

AU - Yamasaki, Ryota

AU - Wood, Thomas Keith

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Bacteria are often thought of as having two dormant phenotypes: the viable but non-culturable (VBNC) state and the persister state. Here we investigate the relatedness of the two stress-induced phenotypes at the single-cell level and examine cell morphology and quantify cell resuscitation. Using the classic starvation conditions to create VBNC cells, we found that the majority of the remaining Escherichia coli population are spherical, have empty cytosol and fail to resuscitate; however, some of the spherical cells resuscitate immediately (most probably those with dense cytosol). Critically, all the culturable cells in this starved population became persister cells within 14 days of starvation. We found that the persister cells initially are rod-like, have clear but limited membrane damage, can resuscitate immediately and gradually become spherical by aging. After 24 h, only rod-shaped persister cells survive, and all the spherical cells lyse. Both cell populations formed under the VBNC-inducing conditions and the persister conditions are metabolically inactive. Therefore, the bacterial population consists of dead cells and persister cells in the VBNC-inducing conditions; that is, the non-lysed particles that do not resuscitate are dead, and the dormant cells that resuscitate are persister cells. Hence, ‘VBNC’ and ‘persister’ describe the same dormant phenotype.

AB - Bacteria are often thought of as having two dormant phenotypes: the viable but non-culturable (VBNC) state and the persister state. Here we investigate the relatedness of the two stress-induced phenotypes at the single-cell level and examine cell morphology and quantify cell resuscitation. Using the classic starvation conditions to create VBNC cells, we found that the majority of the remaining Escherichia coli population are spherical, have empty cytosol and fail to resuscitate; however, some of the spherical cells resuscitate immediately (most probably those with dense cytosol). Critically, all the culturable cells in this starved population became persister cells within 14 days of starvation. We found that the persister cells initially are rod-like, have clear but limited membrane damage, can resuscitate immediately and gradually become spherical by aging. After 24 h, only rod-shaped persister cells survive, and all the spherical cells lyse. Both cell populations formed under the VBNC-inducing conditions and the persister conditions are metabolically inactive. Therefore, the bacterial population consists of dead cells and persister cells in the VBNC-inducing conditions; that is, the non-lysed particles that do not resuscitate are dead, and the dormant cells that resuscitate are persister cells. Hence, ‘VBNC’ and ‘persister’ describe the same dormant phenotype.

UR - http://www.scopus.com/inward/record.url?scp=85044973303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044973303&partnerID=8YFLogxK

U2 - 10.1111/1462-2920.14075

DO - 10.1111/1462-2920.14075

M3 - Article

C2 - 29457686

AN - SCOPUS:85044973303

VL - 20

SP - 2038

EP - 2048

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 6

ER -