Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea

Maria Hoffmann, Steven R. Monday, Marc W. Allard, Errol A. Strain, Paul Whittaker, Marianna Naum, Peter J. Mccarthy, Jose V. Lopez, Markus Fischer, Eric W. Brown

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33 Scopus citations

Abstract

A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rodshaped bacterium, strain N384T, was isolated from a marine sponge (Scleritoderma cyanea; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Curaçao. On the basis of 16S rRNA gene sequencing, strain N384T was shown to belong to the genus Vibrio, most closely related to Vibrio brasiliensis LMG 20546T (98.8%similarity), Vibrio nigripulchritudo ATCC 27043T (98.5 %), Vibrio tubiashii ATCC 19109T (98.6 %) and V. sinaloensis DSM 21326T (98.2 %). The DNA G+C content of strain N384T was 41.6 mol%. An analysis of concatenated sequences of five genes (gyrB, rpoA, pyrH, mreB and ftsZ; 4068 bp) demonstrated a clear separation between strain N384T and its closest neighbours and clustered strain N384T into the 'Orientalis' clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L-/O-) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C13: 0, iso-C15: 0, C15: 0, iso-C16: 0, C16: 0, iso-C17: 0, C17: 1ω8c and C17: 0 were significantly different from those found in V. brasiliensis and V. sinaloensis. The results of DNA-DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus Vibrio. Collectively, these findings confirm that strain N384T represents a novel Vibrio species, for which the name Vibrio caribbeanicus sp. nov. is proposed, with the type strain N384T (=ATCC BAA-2122T=DSM 23640T).

Original languageEnglish (US)
Pages (from-to)1736-1743
Number of pages8
JournalInternational journal of systematic and evolutionary microbiology
Volume62
Issue number8
DOIs
StatePublished - Aug 1 2012

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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