Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea

Maria Hoffmann, Steven R. Monday, Marc W. Allard, Errol A. Strain, Paul Whittaker, Marianna Naum, Peter J. Mccarthy, Jose V. Lopez, Markus Fischer, Eric W. Brown

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rodshaped bacterium, strain N384T, was isolated from a marine sponge (Scleritoderma cyanea; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Curaçao. On the basis of 16S rRNA gene sequencing, strain N384T was shown to belong to the genus Vibrio, most closely related to Vibrio brasiliensis LMG 20546T (98.8%similarity), Vibrio nigripulchritudo ATCC 27043T (98.5 %), Vibrio tubiashii ATCC 19109T (98.6 %) and V. sinaloensis DSM 21326T (98.2 %). The DNA G+C content of strain N384T was 41.6 mol%. An analysis of concatenated sequences of five genes (gyrB, rpoA, pyrH, mreB and ftsZ; 4068 bp) demonstrated a clear separation between strain N384T and its closest neighbours and clustered strain N384T into the 'Orientalis' clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L-/O-) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C13: 0, iso-C15: 0, C15: 0, iso-C16: 0, C16: 0, iso-C17: 0, C17: 1ω8c and C17: 0 were significantly different from those found in V. brasiliensis and V. sinaloensis. The results of DNA-DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus Vibrio. Collectively, these findings confirm that strain N384T represents a novel Vibrio species, for which the name Vibrio caribbeanicus sp. nov. is proposed, with the type strain N384T (=ATCC BAA-2122T=DSM 23640T).

Original languageEnglish (US)
Pages (from-to)1736-1743
Number of pages8
JournalInternational journal of systematic and evolutionary microbiology
Volume62
Issue number8
DOIs
StatePublished - Aug 1 2012

Fingerprint

Vibrio
Porifera
sponge
new species
DNA
gene
lysine decarboxylase
Vibrio orientalis
Vibrio nigripulchritudo
Vibrio tubiashii
fatty acid
arginine deiminase
ornithine decarboxylase
bacterium
Ornithine Decarboxylase
Vibrio caribbeanicus
coast
Base Composition
nucleic acid hybridization
rRNA Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Hoffmann, M., Monday, S. R., Allard, M. W., Strain, E. A., Whittaker, P., Naum, M., ... Brown, E. W. (2012). Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea. International journal of systematic and evolutionary microbiology, 62(8), 1736-1743. https://doi.org/10.1099/ijs.0.032375-0
Hoffmann, Maria ; Monday, Steven R. ; Allard, Marc W. ; Strain, Errol A. ; Whittaker, Paul ; Naum, Marianna ; Mccarthy, Peter J. ; Lopez, Jose V. ; Fischer, Markus ; Brown, Eric W. / Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea. In: International journal of systematic and evolutionary microbiology. 2012 ; Vol. 62, No. 8. pp. 1736-1743.
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abstract = "A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rodshaped bacterium, strain N384T, was isolated from a marine sponge (Scleritoderma cyanea; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Cura{\cc}ao. On the basis of 16S rRNA gene sequencing, strain N384T was shown to belong to the genus Vibrio, most closely related to Vibrio brasiliensis LMG 20546T (98.8{\%}similarity), Vibrio nigripulchritudo ATCC 27043T (98.5 {\%}), Vibrio tubiashii ATCC 19109T (98.6 {\%}) and V. sinaloensis DSM 21326T (98.2 {\%}). The DNA G+C content of strain N384T was 41.6 mol{\%}. An analysis of concatenated sequences of five genes (gyrB, rpoA, pyrH, mreB and ftsZ; 4068 bp) demonstrated a clear separation between strain N384T and its closest neighbours and clustered strain N384T into the 'Orientalis' clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L-/O-) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C13: 0, iso-C15: 0, C15: 0, iso-C16: 0, C16: 0, iso-C17: 0, C17: 1ω8c and C17: 0 were significantly different from those found in V. brasiliensis and V. sinaloensis. The results of DNA-DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus Vibrio. Collectively, these findings confirm that strain N384T represents a novel Vibrio species, for which the name Vibrio caribbeanicus sp. nov. is proposed, with the type strain N384T (=ATCC BAA-2122T=DSM 23640T).",
author = "Maria Hoffmann and Monday, {Steven R.} and Allard, {Marc W.} and Strain, {Errol A.} and Paul Whittaker and Marianna Naum and Mccarthy, {Peter J.} and Lopez, {Jose V.} and Markus Fischer and Brown, {Eric W.}",
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Hoffmann, M, Monday, SR, Allard, MW, Strain, EA, Whittaker, P, Naum, M, Mccarthy, PJ, Lopez, JV, Fischer, M & Brown, EW 2012, 'Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea', International journal of systematic and evolutionary microbiology, vol. 62, no. 8, pp. 1736-1743. https://doi.org/10.1099/ijs.0.032375-0

Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea. / Hoffmann, Maria; Monday, Steven R.; Allard, Marc W.; Strain, Errol A.; Whittaker, Paul; Naum, Marianna; Mccarthy, Peter J.; Lopez, Jose V.; Fischer, Markus; Brown, Eric W.

In: International journal of systematic and evolutionary microbiology, Vol. 62, No. 8, 01.08.2012, p. 1736-1743.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea

AU - Hoffmann, Maria

AU - Monday, Steven R.

AU - Allard, Marc W.

AU - Strain, Errol A.

AU - Whittaker, Paul

AU - Naum, Marianna

AU - Mccarthy, Peter J.

AU - Lopez, Jose V.

AU - Fischer, Markus

AU - Brown, Eric W.

PY - 2012/8/1

Y1 - 2012/8/1

N2 - A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rodshaped bacterium, strain N384T, was isolated from a marine sponge (Scleritoderma cyanea; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Curaçao. On the basis of 16S rRNA gene sequencing, strain N384T was shown to belong to the genus Vibrio, most closely related to Vibrio brasiliensis LMG 20546T (98.8%similarity), Vibrio nigripulchritudo ATCC 27043T (98.5 %), Vibrio tubiashii ATCC 19109T (98.6 %) and V. sinaloensis DSM 21326T (98.2 %). The DNA G+C content of strain N384T was 41.6 mol%. An analysis of concatenated sequences of five genes (gyrB, rpoA, pyrH, mreB and ftsZ; 4068 bp) demonstrated a clear separation between strain N384T and its closest neighbours and clustered strain N384T into the 'Orientalis' clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L-/O-) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C13: 0, iso-C15: 0, C15: 0, iso-C16: 0, C16: 0, iso-C17: 0, C17: 1ω8c and C17: 0 were significantly different from those found in V. brasiliensis and V. sinaloensis. The results of DNA-DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus Vibrio. Collectively, these findings confirm that strain N384T represents a novel Vibrio species, for which the name Vibrio caribbeanicus sp. nov. is proposed, with the type strain N384T (=ATCC BAA-2122T=DSM 23640T).

AB - A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rodshaped bacterium, strain N384T, was isolated from a marine sponge (Scleritoderma cyanea; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Curaçao. On the basis of 16S rRNA gene sequencing, strain N384T was shown to belong to the genus Vibrio, most closely related to Vibrio brasiliensis LMG 20546T (98.8%similarity), Vibrio nigripulchritudo ATCC 27043T (98.5 %), Vibrio tubiashii ATCC 19109T (98.6 %) and V. sinaloensis DSM 21326T (98.2 %). The DNA G+C content of strain N384T was 41.6 mol%. An analysis of concatenated sequences of five genes (gyrB, rpoA, pyrH, mreB and ftsZ; 4068 bp) demonstrated a clear separation between strain N384T and its closest neighbours and clustered strain N384T into the 'Orientalis' clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L-/O-) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C13: 0, iso-C15: 0, C15: 0, iso-C16: 0, C16: 0, iso-C17: 0, C17: 1ω8c and C17: 0 were significantly different from those found in V. brasiliensis and V. sinaloensis. The results of DNA-DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus Vibrio. Collectively, these findings confirm that strain N384T represents a novel Vibrio species, for which the name Vibrio caribbeanicus sp. nov. is proposed, with the type strain N384T (=ATCC BAA-2122T=DSM 23640T).

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