@article{d3d3e7018ac84d968285846e25449eb4,
title = "Viral forensic genomics reveals the relatedness of classic herpes simplex virus strains KOS, KOS63, and KOS79",
abstract = "Herpes simplex virus 1 (HSV-1) is a widespread global pathogen, of which the strain KOS is one of the most extensively studied. Previous sequence studies revealed that KOS does not cluster with other strains of North American geographic origin, but instead clustered with Asian strains. We sequenced a historical isolate of the original KOS strain, called KOS63, along with a separately isolated strain attributed to the same source individual, termed KOS79. Genomic analyses revealed that KOS63 closely resembled other recently sequenced isolates of KOS and was of Asian origin, but that KOS79 was a genetically unrelated strain that clustered in genetic distance analyses with HSV-1 strains of North American/European origin. These data suggest that the human source of KOS63 and KOS79 could have been infected with two genetically unrelated strains of disparate geographic origins. A PCR RFLP test was developed for rapid identification of these strains.",
author = "Bowen, {Christopher D.} and Renner, {Daniel W.} and Shreve, {Jacob T.} and Yolanda Tafuri and Payne, {Kimberly M.} and Dix, {Richard D.} and Kinchington, {Paul R.} and Derek Gatherer and Szpara, {Moriah L.}",
note = "Funding Information: We dedicate this work to Dr. Kendall O. Smith, whose early contributions and mentoring of herpes virologists have led to many subsequent discoveries. This work was supported by Grants to MLS from K22 AI095384/AI/NIAID NIH HHS/United States and the Virus Pathogens Resource (ViPR) Bioinformatics Resource Center . PRK was supported by NIH EY015291 , EY08098 , and the Eye & Ear Institute of Pittsburgh and Research to Prevent Blindness Inc. A portion of this project used the University of Pittsburgh Cancer Institute Genomics Core facility, supported in part by award P30CA047904. The authors thank L.W. Enquist, in whose lab this work was initiated, and members of the Enquist lab for their feedback during early phases of this work. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = may,
day = "1",
doi = "10.1016/j.virol.2016.02.013",
language = "English (US)",
volume = "492",
pages = "179--186",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
}