Visualization of a missing link in retrovirus capsid assembly

Giovanni Cardone, John G. Purdy, Naiqian Cheng, Rebecca Craven, Alasdair C. Steven

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

For a retrovirus such as HIV to be infectious, a properly formed capsid is needed; however, unusually among viruses, retrovirus capsids are highly variable in structure. According to the fullerene conjecture, they are composed of hexamers and pentamers of capsid protein (CA), with the shape of a capsid varying according to how the twelve pentamers are distributed and its size depending on the number of hexamers. Hexamers have been studied in planar and tubular arrays, but the predicted pentamers have not been observed. Here we report cryo-electron microscopic analyses of two in-vitro-assembled capsids of Rous sarcoma virus. Both are icosahedrally symmetric: one is composed of 12 pentamers, and the other of 12 pentamers and 20 hexamers. Fitting of atomic models of the two CA domains into the reconstructions shows three distinct inter-subunit interactions. These observations substantiate the fullerene conjecture, show how pentamers are accommodated at vertices, support the inference that nucleation is a crucial morphologic determinant, and imply that electrostatic interactions govern the differential assembly of pentamers and hexamers.

Original languageEnglish (US)
Pages (from-to)694-698
Number of pages5
JournalNature
Volume457
Issue number7230
DOIs
StatePublished - Feb 5 2009

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Capsid
Retroviridae
Fullerenes
Rous sarcoma virus
Capsid Proteins
Static Electricity
HIV
Electrons
Viruses

All Science Journal Classification (ASJC) codes

  • General

Cite this

Cardone, G., Purdy, J. G., Cheng, N., Craven, R., & Steven, A. C. (2009). Visualization of a missing link in retrovirus capsid assembly. Nature, 457(7230), 694-698. https://doi.org/10.1038/nature07724
Cardone, Giovanni ; Purdy, John G. ; Cheng, Naiqian ; Craven, Rebecca ; Steven, Alasdair C. / Visualization of a missing link in retrovirus capsid assembly. In: Nature. 2009 ; Vol. 457, No. 7230. pp. 694-698.
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Cardone, G, Purdy, JG, Cheng, N, Craven, R & Steven, AC 2009, 'Visualization of a missing link in retrovirus capsid assembly', Nature, vol. 457, no. 7230, pp. 694-698. https://doi.org/10.1038/nature07724

Visualization of a missing link in retrovirus capsid assembly. / Cardone, Giovanni; Purdy, John G.; Cheng, Naiqian; Craven, Rebecca; Steven, Alasdair C.

In: Nature, Vol. 457, No. 7230, 05.02.2009, p. 694-698.

Research output: Contribution to journalArticle

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Cardone G, Purdy JG, Cheng N, Craven R, Steven AC. Visualization of a missing link in retrovirus capsid assembly. Nature. 2009 Feb 5;457(7230):694-698. https://doi.org/10.1038/nature07724