Visualization of protein kinases in lymphocytes stimulated to proliferate with concanavalin A or inhibited with A phorbol ester

Anand P. Iyer, Sharon A. Pishak, Marion J. Sniezek, Andrea M. Mastro

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Stimulation of lymphocytes with a mitogenic lectin such as concanavalin A (ConA) results in differentiation and cell division. Among the changes which occur after stimulation are increases in phosphorylation of proteins and in protein kinase activity. We used a high-resolution, nondenaturing gel system to separate and visualize protein kinases in situ. We have clearly identified both autophosphorylating and substrate-dependent kinases. One band of cyclic AMP-dependent kinase activity was significantly enhanced in lectin-stimulated cells. In contrast, treatment of the cells with phorbol ester under conditions which depress stimulation caused a decrease in the activity of one kinase.

Original languageEnglish (US)
Pages (from-to)392-399
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume121
Issue number1
DOIs
StatePublished - May 31 1984

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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