Visualization of the nanoscale pattern of recently-deposited cellulose microfibrils and matrix materials in never-dried primary walls of the onion epidermis

Tian Zhang, Sahar Mahgsoudy-Louyeh, Bernhard R. Tittmann, Daniel J. Cosgrove

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

For more than 10 years epidermal cell layers from onion scales have been used as a model system to study the relationship between cellulose orientation, cell growth and tissue mechanics. To bring such analyses to the nanoscale, we have developed a procedure for preparing epidermal peels of onion scales for atomic force microscopy to visualize the inner surface (closest to the plasma membrane) of the outer epidermal wall, with minimal disturbance and under conditions very close to the native state of the cell wall. The oriented, multilayer distribution of cellulose microfibrils, approximately ~3 nm wide, is readily observed over extended lengths, along with other features such as the distribution of matrix substances between and on top of microfibrils. The microfibril orientation and alignment appear more dispersed in younger scales compared with older scales, consistent with reported values for mechanical and growth anisotropy of whole epidermal sheets. These results open the door to future work to relate cell wall structure at the nm scale with larger-scale tissue properties such as growth and mechanical behaviors and the action of cell wall loosening agents to induce creep of primary cell walls.

Original languageEnglish (US)
Pages (from-to)853-862
Number of pages10
JournalCellulose
Volume21
Issue number2
DOIs
StatePublished - Jan 1 2014

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Cellulose
Visualization
Cells
Tissue
Cell growth
Cell membranes
Atomic force microscopy
Mechanics
Multilayers
Creep
Anisotropy

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

Cite this

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title = "Visualization of the nanoscale pattern of recently-deposited cellulose microfibrils and matrix materials in never-dried primary walls of the onion epidermis",
abstract = "For more than 10 years epidermal cell layers from onion scales have been used as a model system to study the relationship between cellulose orientation, cell growth and tissue mechanics. To bring such analyses to the nanoscale, we have developed a procedure for preparing epidermal peels of onion scales for atomic force microscopy to visualize the inner surface (closest to the plasma membrane) of the outer epidermal wall, with minimal disturbance and under conditions very close to the native state of the cell wall. The oriented, multilayer distribution of cellulose microfibrils, approximately ~3 nm wide, is readily observed over extended lengths, along with other features such as the distribution of matrix substances between and on top of microfibrils. The microfibril orientation and alignment appear more dispersed in younger scales compared with older scales, consistent with reported values for mechanical and growth anisotropy of whole epidermal sheets. These results open the door to future work to relate cell wall structure at the nm scale with larger-scale tissue properties such as growth and mechanical behaviors and the action of cell wall loosening agents to induce creep of primary cell walls.",
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Visualization of the nanoscale pattern of recently-deposited cellulose microfibrils and matrix materials in never-dried primary walls of the onion epidermis. / Zhang, Tian; Mahgsoudy-Louyeh, Sahar; Tittmann, Bernhard R.; Cosgrove, Daniel J.

In: Cellulose, Vol. 21, No. 2, 01.01.2014, p. 853-862.

Research output: Contribution to journalArticle

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