Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo

Christopher C. Norbury, Daniela Malide, James S. Gibbs, Jack R. Bennink, Jonathan W. Yewdell

Research output: Contribution to journalArticlepeer-review

268 Scopus citations

Abstract

The rational design of vaccines that elicit CD8+T cell responses requires knowledge of the identity of the antigen-presenting cell (APC), the location and time of presentation and the nature of the antigen presented by the APC. Here we address these questions for an antigen encoded by a recombinant vaccinia virus. We found that, following local infection, vaccinia virus infected macrophages and dendritic cells in draining lymph nodes. However, only the dendritic cells presented antigen to naïve CD8+ T cells, as determined by direct visualization of sectioned nodes by confocal microscopy. Presentation occurred as rapidly as 6 h after inoculation and quickly declined in parallel with the number of infected cells present in the nodes. These data provide direct evidence that virus-infected APCs prime naïve CD8+T cells in vivo.

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalNature Immunology
Volume3
Issue number3
DOIs
StatePublished - 2002

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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