Visualizing viral assemblies in a nanoscale biosphere

Brian L. Gilmore, Shannon P. Showalter, Madeline J. Dukes, Justin R. Tanner, Andrew C. Demmert, Sarah M. McDonald, Deborah F. Kelly

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

We present a novel microfluidic platform to examine biological assemblies at high-resolution. We have engineered a functionalized chamber that serves as a "nanoscale biosphere" to capture and maintain rotavirus double-layered particles (DLPs) in a liquid environment. The chamber can be inserted into the column of a transmission electron microscope while being completely isolated from the vacuum system. This configuration allowed us to determine the structure of biological complexes at nanometer-resolution within a self-contained vessel. Images of DLPs were used to calculate the first 3D view of macromolecules in solution. We refer to this new fluidic visualization technology as in situ molecular microscopy.

Original languageEnglish (US)
Pages (from-to)216-219
Number of pages4
JournalLab on a Chip
Volume13
Issue number2
DOIs
StatePublished - Jan 21 2013

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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