Vitamin A supplementation increases the uptake of chylomicron retinyl esters into the brain of neonatal rats raised under vitamin A-marginal conditions

Joanna K. Hodges, Libo Tan, Michael Henry Green, A. Catharine Ross

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: The most rapid phase of brain development occurs during the neonatal period. Vitamin A (VA; retinol) iscritical for many aspects of this process, including neurogenesis, synaptic plasticity, learning, and memory formation.However, the metabolism of retinol in the neonatal brain has not been extensively explored.Objective: We examined the uptake of VA into the brain in neonatal rats raised under VA-marginal conditions (controlgroup) and assessed the effect of VA supplementation on the uptake of VA into the brain.Methods: Sprague-Dawley neonatal rats (n = 104) nursed by mothers fed a VA-marginal diet were randomly assigned andtreated on postnatal day 4 with an oral dose of either VA (6 mg retinyl palmitate/g body weight) or canola oil as the control,both of which contained 1.8 μCi [3H]retinol. Pups (n = 4/group at a time) were killed at 13 sampling times from 30 min to24 d after dosing. The uptake of total retinol, chylomicron-associated retinyl esters (REs), and retinol bound to retinolbindingprotein (RBP) was estimated with the use of WinSAAM version 3.0.8.Results: Total retinol mass in the brain was closely dependent on its mass in plasma over time (r = 0.91; P < 0.001). Theuptake of retinol into the brain involved both postprandial chylomicrons and RBP, with RBP delivering most of the retinol inthe control group [0.27 nmol/d (RBP) compared with 0.01 nmol/d (chylomicrons)]. VA supplementation increased thefractional uptake of chylomicron REs from 0.3% to 1.2% of plasma pool/d, decreased that of RBP retinol from 0.5% to0.2% of plasma pool/d, and increased the transfer rate of chylomicron REs from nearly zero to 0.7 nmol/d, causing a daylongelevation in the brain mass of total retinol.Conclusion: Postprandial chylomicrons may be a primary mechanism for delivering a recently ingested large dose of VA tothe brain of neonatal rats raised under VA-marginal conditions.

Original languageEnglish (US)
Pages (from-to)1677-1683
Number of pages7
JournalJournal of Nutrition
Volume146
Issue number9
DOIs
StatePublished - Jan 1 2016

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Chylomicrons
Vitamin A
Esters
Brain
Neuronal Plasticity
Neurogenesis
Sprague Dawley Rats
Body Weight
Learning

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

@article{0f0d24f10ee04e31b5b5598e775dab77,
title = "Vitamin A supplementation increases the uptake of chylomicron retinyl esters into the brain of neonatal rats raised under vitamin A-marginal conditions",
abstract = "Background: The most rapid phase of brain development occurs during the neonatal period. Vitamin A (VA; retinol) iscritical for many aspects of this process, including neurogenesis, synaptic plasticity, learning, and memory formation.However, the metabolism of retinol in the neonatal brain has not been extensively explored.Objective: We examined the uptake of VA into the brain in neonatal rats raised under VA-marginal conditions (controlgroup) and assessed the effect of VA supplementation on the uptake of VA into the brain.Methods: Sprague-Dawley neonatal rats (n = 104) nursed by mothers fed a VA-marginal diet were randomly assigned andtreated on postnatal day 4 with an oral dose of either VA (6 mg retinyl palmitate/g body weight) or canola oil as the control,both of which contained 1.8 μCi [3H]retinol. Pups (n = 4/group at a time) were killed at 13 sampling times from 30 min to24 d after dosing. The uptake of total retinol, chylomicron-associated retinyl esters (REs), and retinol bound to retinolbindingprotein (RBP) was estimated with the use of WinSAAM version 3.0.8.Results: Total retinol mass in the brain was closely dependent on its mass in plasma over time (r = 0.91; P < 0.001). Theuptake of retinol into the brain involved both postprandial chylomicrons and RBP, with RBP delivering most of the retinol inthe control group [0.27 nmol/d (RBP) compared with 0.01 nmol/d (chylomicrons)]. VA supplementation increased thefractional uptake of chylomicron REs from 0.3{\%} to 1.2{\%} of plasma pool/d, decreased that of RBP retinol from 0.5{\%} to0.2{\%} of plasma pool/d, and increased the transfer rate of chylomicron REs from nearly zero to 0.7 nmol/d, causing a daylongelevation in the brain mass of total retinol.Conclusion: Postprandial chylomicrons may be a primary mechanism for delivering a recently ingested large dose of VA tothe brain of neonatal rats raised under VA-marginal conditions.",
author = "Hodges, {Joanna K.} and Libo Tan and Green, {Michael Henry} and Ross, {A. Catharine}",
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pages = "1677--1683",
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Vitamin A supplementation increases the uptake of chylomicron retinyl esters into the brain of neonatal rats raised under vitamin A-marginal conditions. / Hodges, Joanna K.; Tan, Libo; Green, Michael Henry; Ross, A. Catharine.

In: Journal of Nutrition, Vol. 146, No. 9, 01.01.2016, p. 1677-1683.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Vitamin A supplementation increases the uptake of chylomicron retinyl esters into the brain of neonatal rats raised under vitamin A-marginal conditions

AU - Hodges, Joanna K.

AU - Tan, Libo

AU - Green, Michael Henry

AU - Ross, A. Catharine

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background: The most rapid phase of brain development occurs during the neonatal period. Vitamin A (VA; retinol) iscritical for many aspects of this process, including neurogenesis, synaptic plasticity, learning, and memory formation.However, the metabolism of retinol in the neonatal brain has not been extensively explored.Objective: We examined the uptake of VA into the brain in neonatal rats raised under VA-marginal conditions (controlgroup) and assessed the effect of VA supplementation on the uptake of VA into the brain.Methods: Sprague-Dawley neonatal rats (n = 104) nursed by mothers fed a VA-marginal diet were randomly assigned andtreated on postnatal day 4 with an oral dose of either VA (6 mg retinyl palmitate/g body weight) or canola oil as the control,both of which contained 1.8 μCi [3H]retinol. Pups (n = 4/group at a time) were killed at 13 sampling times from 30 min to24 d after dosing. The uptake of total retinol, chylomicron-associated retinyl esters (REs), and retinol bound to retinolbindingprotein (RBP) was estimated with the use of WinSAAM version 3.0.8.Results: Total retinol mass in the brain was closely dependent on its mass in plasma over time (r = 0.91; P < 0.001). Theuptake of retinol into the brain involved both postprandial chylomicrons and RBP, with RBP delivering most of the retinol inthe control group [0.27 nmol/d (RBP) compared with 0.01 nmol/d (chylomicrons)]. VA supplementation increased thefractional uptake of chylomicron REs from 0.3% to 1.2% of plasma pool/d, decreased that of RBP retinol from 0.5% to0.2% of plasma pool/d, and increased the transfer rate of chylomicron REs from nearly zero to 0.7 nmol/d, causing a daylongelevation in the brain mass of total retinol.Conclusion: Postprandial chylomicrons may be a primary mechanism for delivering a recently ingested large dose of VA tothe brain of neonatal rats raised under VA-marginal conditions.

AB - Background: The most rapid phase of brain development occurs during the neonatal period. Vitamin A (VA; retinol) iscritical for many aspects of this process, including neurogenesis, synaptic plasticity, learning, and memory formation.However, the metabolism of retinol in the neonatal brain has not been extensively explored.Objective: We examined the uptake of VA into the brain in neonatal rats raised under VA-marginal conditions (controlgroup) and assessed the effect of VA supplementation on the uptake of VA into the brain.Methods: Sprague-Dawley neonatal rats (n = 104) nursed by mothers fed a VA-marginal diet were randomly assigned andtreated on postnatal day 4 with an oral dose of either VA (6 mg retinyl palmitate/g body weight) or canola oil as the control,both of which contained 1.8 μCi [3H]retinol. Pups (n = 4/group at a time) were killed at 13 sampling times from 30 min to24 d after dosing. The uptake of total retinol, chylomicron-associated retinyl esters (REs), and retinol bound to retinolbindingprotein (RBP) was estimated with the use of WinSAAM version 3.0.8.Results: Total retinol mass in the brain was closely dependent on its mass in plasma over time (r = 0.91; P < 0.001). Theuptake of retinol into the brain involved both postprandial chylomicrons and RBP, with RBP delivering most of the retinol inthe control group [0.27 nmol/d (RBP) compared with 0.01 nmol/d (chylomicrons)]. VA supplementation increased thefractional uptake of chylomicron REs from 0.3% to 1.2% of plasma pool/d, decreased that of RBP retinol from 0.5% to0.2% of plasma pool/d, and increased the transfer rate of chylomicron REs from nearly zero to 0.7 nmol/d, causing a daylongelevation in the brain mass of total retinol.Conclusion: Postprandial chylomicrons may be a primary mechanism for delivering a recently ingested large dose of VA tothe brain of neonatal rats raised under VA-marginal conditions.

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