Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors

Ehsan Mahdinia, Ali Demirci, Aydin Berenjian

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

Menaquinone-7 (MK-7) is a subtype of Vitamin K which plays a key role in promoting heart and bone health. MK-7 can be excreted by many strains of bacteria. Among these strains, Bacillus subtilis natto seems to hold a key position. On the other hand, Bacillus subtilis, especially in static fermentations, tends to form pellicles and possibly biofilms under suitable conditions which promote MK-7 secretion but at the same time create operational and scaling up issues during fermentation. Therefore, biofilm reactors seem to be a viable strategy to create a controlled environment for the bacteria to form biofilm and simultaneously address operational and scaling up issues through robust agitation and aeration. The present study aims at using a suitable Plastic Composite Support (PCS) and Bacillus strain to optimize the media components and growth conditions in a glucose-based medium using a biofilm reactor. The optimum conditions were found as 30°C, 234 rpm and pH 6.48, and the optimum composition was of glucose (152.6 g/L), yeast extract (8 g/L) and casein (17.6 g/L) yielding a maximum MK-7 concentration of 20.46±0.51 mg/L in batch biofilm reactors Furthermore, MK-7 concentrations were enhanced using a fed-batch strategy to concentrations as high as 28.7±1.1 mg/L, which was 40.3% higher as compared to the batch process.

Original languageEnglish (US)
DOIs
StatePublished - Jan 1 2018
EventASABE 2018 Annual International Meeting - Detroit, United States
Duration: Jul 29 2018Aug 1 2018

Other

OtherASABE 2018 Annual International Meeting
CountryUnited States
CityDetroit
Period7/29/188/1/18

Fingerprint

natto
Vitamin K 2
Vitamins
menaquinones
Biofilms
Bacilli
Bacillus subtilis
biofilm
Glucose
glucose
Fermentation
Bacteria
fermentation
Casein
vitamin K
Vitamin K
bacteria
composite materials
Bacillus (bacteria)
agitation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Agronomy and Crop Science

Cite this

Mahdinia, E., Demirci, A., & Berenjian, A. (2018). Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors. Paper presented at ASABE 2018 Annual International Meeting, Detroit, United States. https://doi.org/10.13031/aim.201800333
Mahdinia, Ehsan ; Demirci, Ali ; Berenjian, Aydin. / Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors. Paper presented at ASABE 2018 Annual International Meeting, Detroit, United States.
@conference{de146cf86f11482d94c0e093260d9671,
title = "Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors",
abstract = "Menaquinone-7 (MK-7) is a subtype of Vitamin K which plays a key role in promoting heart and bone health. MK-7 can be excreted by many strains of bacteria. Among these strains, Bacillus subtilis natto seems to hold a key position. On the other hand, Bacillus subtilis, especially in static fermentations, tends to form pellicles and possibly biofilms under suitable conditions which promote MK-7 secretion but at the same time create operational and scaling up issues during fermentation. Therefore, biofilm reactors seem to be a viable strategy to create a controlled environment for the bacteria to form biofilm and simultaneously address operational and scaling up issues through robust agitation and aeration. The present study aims at using a suitable Plastic Composite Support (PCS) and Bacillus strain to optimize the media components and growth conditions in a glucose-based medium using a biofilm reactor. The optimum conditions were found as 30°C, 234 rpm and pH 6.48, and the optimum composition was of glucose (152.6 g/L), yeast extract (8 g/L) and casein (17.6 g/L) yielding a maximum MK-7 concentration of 20.46±0.51 mg/L in batch biofilm reactors Furthermore, MK-7 concentrations were enhanced using a fed-batch strategy to concentrations as high as 28.7±1.1 mg/L, which was 40.3{\%} higher as compared to the batch process.",
author = "Ehsan Mahdinia and Ali Demirci and Aydin Berenjian",
year = "2018",
month = "1",
day = "1",
doi = "10.13031/aim.201800333",
language = "English (US)",
note = "ASABE 2018 Annual International Meeting ; Conference date: 29-07-2018 Through 01-08-2018",

}

Mahdinia, E, Demirci, A & Berenjian, A 2018, 'Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors', Paper presented at ASABE 2018 Annual International Meeting, Detroit, United States, 7/29/18 - 8/1/18. https://doi.org/10.13031/aim.201800333

Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors. / Mahdinia, Ehsan; Demirci, Ali; Berenjian, Aydin.

2018. Paper presented at ASABE 2018 Annual International Meeting, Detroit, United States.

Research output: Contribution to conferencePaper

TY - CONF

T1 - Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors

AU - Mahdinia, Ehsan

AU - Demirci, Ali

AU - Berenjian, Aydin

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Menaquinone-7 (MK-7) is a subtype of Vitamin K which plays a key role in promoting heart and bone health. MK-7 can be excreted by many strains of bacteria. Among these strains, Bacillus subtilis natto seems to hold a key position. On the other hand, Bacillus subtilis, especially in static fermentations, tends to form pellicles and possibly biofilms under suitable conditions which promote MK-7 secretion but at the same time create operational and scaling up issues during fermentation. Therefore, biofilm reactors seem to be a viable strategy to create a controlled environment for the bacteria to form biofilm and simultaneously address operational and scaling up issues through robust agitation and aeration. The present study aims at using a suitable Plastic Composite Support (PCS) and Bacillus strain to optimize the media components and growth conditions in a glucose-based medium using a biofilm reactor. The optimum conditions were found as 30°C, 234 rpm and pH 6.48, and the optimum composition was of glucose (152.6 g/L), yeast extract (8 g/L) and casein (17.6 g/L) yielding a maximum MK-7 concentration of 20.46±0.51 mg/L in batch biofilm reactors Furthermore, MK-7 concentrations were enhanced using a fed-batch strategy to concentrations as high as 28.7±1.1 mg/L, which was 40.3% higher as compared to the batch process.

AB - Menaquinone-7 (MK-7) is a subtype of Vitamin K which plays a key role in promoting heart and bone health. MK-7 can be excreted by many strains of bacteria. Among these strains, Bacillus subtilis natto seems to hold a key position. On the other hand, Bacillus subtilis, especially in static fermentations, tends to form pellicles and possibly biofilms under suitable conditions which promote MK-7 secretion but at the same time create operational and scaling up issues during fermentation. Therefore, biofilm reactors seem to be a viable strategy to create a controlled environment for the bacteria to form biofilm and simultaneously address operational and scaling up issues through robust agitation and aeration. The present study aims at using a suitable Plastic Composite Support (PCS) and Bacillus strain to optimize the media components and growth conditions in a glucose-based medium using a biofilm reactor. The optimum conditions were found as 30°C, 234 rpm and pH 6.48, and the optimum composition was of glucose (152.6 g/L), yeast extract (8 g/L) and casein (17.6 g/L) yielding a maximum MK-7 concentration of 20.46±0.51 mg/L in batch biofilm reactors Furthermore, MK-7 concentrations were enhanced using a fed-batch strategy to concentrations as high as 28.7±1.1 mg/L, which was 40.3% higher as compared to the batch process.

UR - http://www.scopus.com/inward/record.url?scp=85053542785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053542785&partnerID=8YFLogxK

U2 - 10.13031/aim.201800333

DO - 10.13031/aim.201800333

M3 - Paper

AN - SCOPUS:85053542785

ER -

Mahdinia E, Demirci A, Berenjian A. Vitamin K2 (Menaquinone-7) production by Bacillus subtilis natto by using a glucose-based medium in biofilm reactors. 2018. Paper presented at ASABE 2018 Annual International Meeting, Detroit, United States. https://doi.org/10.13031/aim.201800333