Watching the bacteriophage N4 RNA polymerase transcription by time-dependent soak-trigger-freeze x-ray crystallography

Ritwika S. Basu, Katsuhiko Murakami

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: The two-metal-ion mechanism of the nucleotidyl transfer reaction by RNA/DNA polymerases has not been adequately elucidated due to lack of temporal resolution. Results: Soak-trigger-freeze x-ray crystallography revealed structures of natural, time-resolved intermediates of transcript initiation. Conclusion: First structural evidence shows that catalytic metal binds after the nucleotide and nucleotide-binding metal and right before reaction chemistry. Significance: Time-dependent soak-trigger-freeze x-ray crystallography can yield functionally relevant high resolution information to study enzyme reactions.

Original languageEnglish (US)
Pages (from-to)3305-3311
Number of pages7
JournalJournal of Biological Chemistry
Volume288
Issue number5
DOIs
StatePublished - Feb 1 2013

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Bacteriophage N4
Crystallography
Bacteriophages
DNA-Directed RNA Polymerases
Transcription
Nucleotides
Metals
X-Rays
X rays
DNA-Directed DNA Polymerase
Metal ions
RNA
Transfer RNA
Enzymes
Ions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Watching the bacteriophage N4 RNA polymerase transcription by time-dependent soak-trigger-freeze x-ray crystallography. / Basu, Ritwika S.; Murakami, Katsuhiko.

In: Journal of Biological Chemistry, Vol. 288, No. 5, 01.02.2013, p. 3305-3311.

Research output: Contribution to journalArticle

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AB - Background: The two-metal-ion mechanism of the nucleotidyl transfer reaction by RNA/DNA polymerases has not been adequately elucidated due to lack of temporal resolution. Results: Soak-trigger-freeze x-ray crystallography revealed structures of natural, time-resolved intermediates of transcript initiation. Conclusion: First structural evidence shows that catalytic metal binds after the nucleotide and nucleotide-binding metal and right before reaction chemistry. Significance: Time-dependent soak-trigger-freeze x-ray crystallography can yield functionally relevant high resolution information to study enzyme reactions.

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