White Button mushrooms increase microbialdiversity and accelerate the resolution of Citrobacter rodentium infection in mice

Jyotika Varshney, Jot Hui Ooi, Bhushan M. Jayarao, Istvan Albert, Jenny Fisher, Rhonda L. Smith, Andrew D. Patterson, Margherita T. Cantorna

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of feeding C57BL/6 mice white button (WB) mushrooms or control (CTRL) diets for 6 wk was determined on the bacterial microflora, urinary metabolome, and resistance to a gastrointestinal (GI) pathogen. Feedingmice a diet containing 1 g WB mushrooms/100 g diet resulted in changes in the microflora that were evident at 2 wk and stabilized after 4 wk of WB feeding. Compared with CTRL-fed mice, WB feeding (1 g/100 g diet) increased the diversity of the microflora and reduced potentially pathogenic (e.g., Clostridia) bacteria in the GI tract. Bacteria from the Bacteroidetes phylum increased and the Firmicutes phylum decreased in mushroom-fed mice compared with CTRL. The changes in the microflora were also reflected in the urinary metabolome that showed a metabolicshift in the WB-fed compared with the CTRL-fed mice. The WB feeding and changes in the microbiome were associated with fewer inflammatory cells and decreased colitis severity in the GI mucosa following Citrobacter rodentium infection compared with CTRL. Paradoxically, the clearance of C. rodentium infection did not differ even though Ifn-γ and Il-17 were higher in the colons of the WB-fed mice compared with CTRL. Adding modest amounts of WB mushrooms (1 g/100 g diet) to the diet changed the composition of the normal flora and the urinary metabolome of mice and these changes resulted in better control of inflammation and resolution of infection with C.rodentium.

Original languageEnglish (US)
Pages (from-to)526-532
Number of pages7
JournalJournal of Nutrition
Volume143
Issue number4
DOIs
StatePublished - Apr 1 2013

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Citrobacter rodentium
Agaricales
Diet
Metabolome
Infection
Bacteroidetes
Bacteria
Clostridium
Microbiota
Colitis
Inbred C57BL Mouse
Gastrointestinal Tract
Colon
Mucous Membrane
Inflammation

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

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title = "White Button mushrooms increase microbialdiversity and accelerate the resolution of Citrobacter rodentium infection in mice",
abstract = "The effect of feeding C57BL/6 mice white button (WB) mushrooms or control (CTRL) diets for 6 wk was determined on the bacterial microflora, urinary metabolome, and resistance to a gastrointestinal (GI) pathogen. Feedingmice a diet containing 1 g WB mushrooms/100 g diet resulted in changes in the microflora that were evident at 2 wk and stabilized after 4 wk of WB feeding. Compared with CTRL-fed mice, WB feeding (1 g/100 g diet) increased the diversity of the microflora and reduced potentially pathogenic (e.g., Clostridia) bacteria in the GI tract. Bacteria from the Bacteroidetes phylum increased and the Firmicutes phylum decreased in mushroom-fed mice compared with CTRL. The changes in the microflora were also reflected in the urinary metabolome that showed a metabolicshift in the WB-fed compared with the CTRL-fed mice. The WB feeding and changes in the microbiome were associated with fewer inflammatory cells and decreased colitis severity in the GI mucosa following Citrobacter rodentium infection compared with CTRL. Paradoxically, the clearance of C. rodentium infection did not differ even though Ifn-γ and Il-17 were higher in the colons of the WB-fed mice compared with CTRL. Adding modest amounts of WB mushrooms (1 g/100 g diet) to the diet changed the composition of the normal flora and the urinary metabolome of mice and these changes resulted in better control of inflammation and resolution of infection with C.rodentium.",
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White Button mushrooms increase microbialdiversity and accelerate the resolution of Citrobacter rodentium infection in mice. / Varshney, Jyotika; Ooi, Jot Hui; Jayarao, Bhushan M.; Albert, Istvan; Fisher, Jenny; Smith, Rhonda L.; Patterson, Andrew D.; Cantorna, Margherita T.

In: Journal of Nutrition, Vol. 143, No. 4, 01.04.2013, p. 526-532.

Research output: Contribution to journalArticle

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T1 - White Button mushrooms increase microbialdiversity and accelerate the resolution of Citrobacter rodentium infection in mice

AU - Varshney, Jyotika

AU - Ooi, Jot Hui

AU - Jayarao, Bhushan M.

AU - Albert, Istvan

AU - Fisher, Jenny

AU - Smith, Rhonda L.

AU - Patterson, Andrew D.

AU - Cantorna, Margherita T.

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N2 - The effect of feeding C57BL/6 mice white button (WB) mushrooms or control (CTRL) diets for 6 wk was determined on the bacterial microflora, urinary metabolome, and resistance to a gastrointestinal (GI) pathogen. Feedingmice a diet containing 1 g WB mushrooms/100 g diet resulted in changes in the microflora that were evident at 2 wk and stabilized after 4 wk of WB feeding. Compared with CTRL-fed mice, WB feeding (1 g/100 g diet) increased the diversity of the microflora and reduced potentially pathogenic (e.g., Clostridia) bacteria in the GI tract. Bacteria from the Bacteroidetes phylum increased and the Firmicutes phylum decreased in mushroom-fed mice compared with CTRL. The changes in the microflora were also reflected in the urinary metabolome that showed a metabolicshift in the WB-fed compared with the CTRL-fed mice. The WB feeding and changes in the microbiome were associated with fewer inflammatory cells and decreased colitis severity in the GI mucosa following Citrobacter rodentium infection compared with CTRL. Paradoxically, the clearance of C. rodentium infection did not differ even though Ifn-γ and Il-17 were higher in the colons of the WB-fed mice compared with CTRL. Adding modest amounts of WB mushrooms (1 g/100 g diet) to the diet changed the composition of the normal flora and the urinary metabolome of mice and these changes resulted in better control of inflammation and resolution of infection with C.rodentium.

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