Whole-brain mapping of neuronal activity in the learned helplessness model of depression

Yongsoo Kim, Zinaida Perova, Martine M. Mirrione, Kith Pradhan, Fritz A. Henn, Stephen Shea, Pavel Osten, Bo Li

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Some individuals are resilient, whereas others succumb to despair in repeated stressful situations. The neurobiological mechanisms underlying such divergent behavioral responses remain unclear. Here, we employed an automated method for mapping neuronal activity in search of signatures of stress responses in the entire mouse brain. We used serial two-photon tomography to detect expression of c-FosGFP – a marker of neuronal activation – in c-fosGFP transgenic mice subjected to the learned helplessness (LH) procedure, a widely used model of stress-induced depression-like phenotype in laboratory animals. We found that mice showing “helpless” behavior had an overall brain-wide reduction in the level of neuronal activation compared with mice showing “resilient” behavior, with the exception of a few brain areas, including the locus coeruleus, that were more activated in the helpless mice. In addition, the helpless mice showed a strong trend of having higher similarity in whole-brain activity profile among individuals, suggesting that helplessness is represented by a more stereotypic brain-wide activation pattern. This latter effect was confirmed in rats subjected to the LH procedure, using 2-deoxy-2[18F]fluoro-D-glucose positron emission tomography to assess neural activity. Our findings reveal distinct brain activity markings that correlate with adaptive and maladaptive behavioral responses to stress, and provide a framework for further studies investigating the contribution of specific brain regions to maladaptive stress responses.

Original languageEnglish (US)
Article number3
JournalFrontiers in Neural Circuits
Volume10
Issue numberFEB
DOIs
StatePublished - Feb 3 2016

Fingerprint

Learned Helplessness
Brain Mapping
Depression
Brain
Locus Coeruleus
Fluorodeoxyglucose F18
Laboratory Animals
Photons
Positron-Emission Tomography
Transgenic Mice
Tomography
Phenotype

All Science Journal Classification (ASJC) codes

  • Neuroscience (miscellaneous)
  • Sensory Systems
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Kim, Yongsoo ; Perova, Zinaida ; Mirrione, Martine M. ; Pradhan, Kith ; Henn, Fritz A. ; Shea, Stephen ; Osten, Pavel ; Li, Bo. / Whole-brain mapping of neuronal activity in the learned helplessness model of depression. In: Frontiers in Neural Circuits. 2016 ; Vol. 10, No. FEB.
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Kim, Y, Perova, Z, Mirrione, MM, Pradhan, K, Henn, FA, Shea, S, Osten, P & Li, B 2016, 'Whole-brain mapping of neuronal activity in the learned helplessness model of depression', Frontiers in Neural Circuits, vol. 10, no. FEB, 3. https://doi.org/10.3389/fncir.2016.00003

Whole-brain mapping of neuronal activity in the learned helplessness model of depression. / Kim, Yongsoo; Perova, Zinaida; Mirrione, Martine M.; Pradhan, Kith; Henn, Fritz A.; Shea, Stephen; Osten, Pavel; Li, Bo.

In: Frontiers in Neural Circuits, Vol. 10, No. FEB, 3, 03.02.2016.

Research output: Contribution to journalArticle

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