Whole-genome comparisons of Penicillium spp. Reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay

Guangxi Wu, Wayne M. Jurick, Franz J. Lichtner, Hui Peng, Guohua Yin, Verneta L. Gaskins, Yanbin Yin, Sui Sheng Hua, Kari Anne Peter, Joan W. Bennett

Research output: Contribution to journalArticle

Abstract

Blue mold is a postharvest rot of pomaceous fruits caused by Penicillium expansum and a number of other Penicillium species. The genome of the highly aggressive P. expansum strain R19 was re-sequenced and analyzed together with the genome of the less aggressive P. solitum strain RS1. Whole genome scale similarities and differences were examined. A phylogenetic analysis of P. expansum, P. solitum, and several closely related Penicillium species revealed that the two pathogens isolated from decayed apple with blue mold symptoms are not each other’s closest relatives. Among a total of 10,560 and 10,672 protein coding sequences respectively, a comparative genomics analysis revealed 41 genes in P. expansum R19 and 43 genes in P. solitum RS1 that are unique to these two species. These genes may be associated with pome fruit–fungal interactions, subsequent decay processes, and mycotoxin accumulation. An intact patulin gene cluster consisting of 15 biosynthetic genes was identified in the patulin producing P. expansum strain R19, while only a remnant, seven-gene cluster was identified in the patulin-deficient P. solitum strain. However, P. solitum contained a large number of additional secondary metabolite gene clusters, indicating that this species has the potential capacity to produce an array of known as well as not-yet-identified products of possible toxicological or biotechnological interest.

Original languageEnglish (US)
Article numbere6170
JournalPeerJ
Volume2019
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Penicillium expansum
Penicillium
Patulin
Malus
Multigene Family
Fruits
multigene family
aggression
Fruit
patulin
apples
Genes
deterioration
Genome
fruits
genome
Fungi
genes
molds (fungi)
Mycotoxins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Wu, Guangxi ; Jurick, Wayne M. ; Lichtner, Franz J. ; Peng, Hui ; Yin, Guohua ; Gaskins, Verneta L. ; Yin, Yanbin ; Hua, Sui Sheng ; Peter, Kari Anne ; Bennett, Joan W. / Whole-genome comparisons of Penicillium spp. Reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay. In: PeerJ. 2019 ; Vol. 2019, No. 1.
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Whole-genome comparisons of Penicillium spp. Reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay. / Wu, Guangxi; Jurick, Wayne M.; Lichtner, Franz J.; Peng, Hui; Yin, Guohua; Gaskins, Verneta L.; Yin, Yanbin; Hua, Sui Sheng; Peter, Kari Anne; Bennett, Joan W.

In: PeerJ, Vol. 2019, No. 1, e6170, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Wu, Guangxi

AU - Jurick, Wayne M.

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AU - Yin, Guohua

AU - Gaskins, Verneta L.

AU - Yin, Yanbin

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AU - Peter, Kari Anne

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