Widespread distribution of knockdown resistance mutations in the bed bug, Cimex lectularius (Hemiptera: Cimicidae), populations in the United States

Fang Zhu, John Wigginton, Alvaro Romero, Ali Moore, Kimberly Ferguson, Roshan Palli, Michael F. Potter, Kenneth F. Haynes, Subba R. Palli

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

We previously reported high deltamethrin resistance in bed bugs, Cimex lectularius, collected from multiple areas of the United States (Romero et al., 2007). Recently, two mutations, the Valine to Leucine mutation (V419L) and the Leucine to Isoleucine mutation (L925I) in voltagegated sodium channel α-subunit gene, had been identified to be responsible for knockdown resistance (kdr) to deltamethrin in bed bugs collected from New York (Yoon et al., 2008). The current study was undertaken to investigate the distribution of these two kdr mutations in 110 bed bug populations collected in the United States. Out of the 17 bed bug populations that were assayed for deltamethrin susceptibility, two resistant populations collected in the Cincinnati area and three deltamethrin-susceptible lab colonies showed neither of the two reported mutations (haplotype A). The remaining 12 populations contained L925I or both V419L and L925I mutations in voltage-gated sodium channel a-subunit gene (haplotypes B&C). In 93 populations that were not assayed for deltamethrin susceptibility, 12 contained neither of the two mutations (haplotype A) and 81 contained L925I or V419L or both mutations (haplotypes B-D). Thus, 88% of the bed bug populations collected showed target-site mutations. These data suggest that deltamethrin resistance conferred by target-site insensitivity of sodium channel is widely spread in bed bug populations across the United States.

Original languageEnglish (US)
Pages (from-to)245-257
Number of pages13
JournalArchives of Insect Biochemistry and Physiology
Volume73
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Cimicidae
Bedbugs
Cimex lectularius
pesticide resistance
Hemiptera
deltamethrin
mutation
Mutation
Population
Haplotypes
sodium channels
Sodium Channels
haplotypes
Leucine
Genes
Voltage-Gated Sodium Channels
leucine
Isoleucine
Valine
decamethrin

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Insect Science

Cite this

Zhu, Fang ; Wigginton, John ; Romero, Alvaro ; Moore, Ali ; Ferguson, Kimberly ; Palli, Roshan ; Potter, Michael F. ; Haynes, Kenneth F. ; Palli, Subba R. / Widespread distribution of knockdown resistance mutations in the bed bug, Cimex lectularius (Hemiptera : Cimicidae), populations in the United States. In: Archives of Insect Biochemistry and Physiology. 2010 ; Vol. 73, No. 4. pp. 245-257.
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abstract = "We previously reported high deltamethrin resistance in bed bugs, Cimex lectularius, collected from multiple areas of the United States (Romero et al., 2007). Recently, two mutations, the Valine to Leucine mutation (V419L) and the Leucine to Isoleucine mutation (L925I) in voltagegated sodium channel α-subunit gene, had been identified to be responsible for knockdown resistance (kdr) to deltamethrin in bed bugs collected from New York (Yoon et al., 2008). The current study was undertaken to investigate the distribution of these two kdr mutations in 110 bed bug populations collected in the United States. Out of the 17 bed bug populations that were assayed for deltamethrin susceptibility, two resistant populations collected in the Cincinnati area and three deltamethrin-susceptible lab colonies showed neither of the two reported mutations (haplotype A). The remaining 12 populations contained L925I or both V419L and L925I mutations in voltage-gated sodium channel a-subunit gene (haplotypes B&C). In 93 populations that were not assayed for deltamethrin susceptibility, 12 contained neither of the two mutations (haplotype A) and 81 contained L925I or V419L or both mutations (haplotypes B-D). Thus, 88{\%} of the bed bug populations collected showed target-site mutations. These data suggest that deltamethrin resistance conferred by target-site insensitivity of sodium channel is widely spread in bed bug populations across the United States.",
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Widespread distribution of knockdown resistance mutations in the bed bug, Cimex lectularius (Hemiptera : Cimicidae), populations in the United States. / Zhu, Fang; Wigginton, John; Romero, Alvaro; Moore, Ali; Ferguson, Kimberly; Palli, Roshan; Potter, Michael F.; Haynes, Kenneth F.; Palli, Subba R.

In: Archives of Insect Biochemistry and Physiology, Vol. 73, No. 4, 01.04.2010, p. 245-257.

Research output: Contribution to journalArticle

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