Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity

David A. Hess, Timothy P. Craft, Louisa Wirthlin, Sarah Hohm, Ping Zhou, William C. Eades, Michael H. Creer, Mark S. Sands, Jan A. Nolta

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Transplanted adult progenitor cells distribute to peripheral organs and can promote endogenous cellular repair in damaged tissues. However, development of cell-based regenerative therapies has been hindered by the lack of preclinical models to efficiently assess multiple organ distribution and difficulty defining human cells with regenerative function. After transplantation into β-glucuronidase (GUSB)-deficient NOD/SCID/mucopolysaccharidosis type VII mice, we characterized the distribution of lineage-depleted human umbilical cord blood-derived cells purified by selection using high aldehyde dehydrogenase (ALDH) activity with CD133 coexpression. ALDHhi or ALDH hiCD133+ cells produced robust hematopoietic reconstitution and variable levels of tissue distribution in multiple organs. GUSB+ donor cells that coexpressed human leukocyte antigen (HLA-A,B,C) and hematopoietic (CD45+) cell surface markers were the primary cell phenotype found adjacent to the vascular beds of several tissues, including islet and ductal regions of mouse pancreata. In contrast, variable phenotypes were detected in the chimeric liver, with HLA+/CD45 + cells demonstrating robust GUSB expression adjacent to blood vessels and CD45-/HLA- cells with diluted GUSB expression predominant in the liver parenchyma. However, true nonhematopoietic human (HLA+/CD45-) cells were rarely detected in other peripheral tissues, suggesting that these GUSB+/HLA -/CD45- cells in the liver were a result of downregulated human surface marker expression in vivo, not widespread seeding of nonhematopoietic cells. However, relying solely on continued expression of cell surface markers, as used in traditional xenotransplantation models, may underestimate true tissue distribution. ALDH-expressing progenitor cells demonstrated widespread and tissue-specific distribution of variable cellular phenotypes, indicating that these adult progenitor cells should be explored in transplantation models of tissue damage.

Original languageEnglish (US)
Pages (from-to)611-620
Number of pages10
JournalSTEM CELLS
Volume26
Issue number3
DOIs
StatePublished - Mar 1 2008

Fingerprint

Aldehyde Dehydrogenase
Tissue Distribution
Stem Cells
Phenotype
Blood Vessels
Liver
Mucopolysaccharidosis VII
Tissue Transplantation
Heterologous Transplantation
HLA-A Antigens
HLA-B Antigens
Glucuronidase
HLA Antigens
Fetal Blood
Pancreas
Blood Cells
Down-Regulation
Transplantation

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Hess, David A. ; Craft, Timothy P. ; Wirthlin, Louisa ; Hohm, Sarah ; Zhou, Ping ; Eades, William C. ; Creer, Michael H. ; Sands, Mark S. ; Nolta, Jan A. / Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity. In: STEM CELLS. 2008 ; Vol. 26, No. 3. pp. 611-620.
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Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity. / Hess, David A.; Craft, Timothy P.; Wirthlin, Louisa; Hohm, Sarah; Zhou, Ping; Eades, William C.; Creer, Michael H.; Sands, Mark S.; Nolta, Jan A.

In: STEM CELLS, Vol. 26, No. 3, 01.03.2008, p. 611-620.

Research output: Contribution to journalArticle

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