Within gut physicochemical variation does not correspond to distinct resident fungal and bacterial communities in the tree-killing xylophage, Anoplophora glabripennis

Charles J. Mason, David C. Long, Elizabeth M. McCarthy, Nivedita Nagachar, Cristina Rosa, Erin D. Scully, Ming Tien, Kelli Hoover

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Insect guts harbor diverse microbial assemblages that can be influenced by multiple factors, including gut physiology and interactions by the host with its environment. The Asian longhorned beetle (A. glabripennis; Cerambycidae: Lamiinae) is an invasive tree–killing insect that harbors a diverse consortium of fungal and bacterial gut associates that provision nutrients and facilitate lignocellulose digestion. The physicochemical conditions of the A. glabripennis gut and how these conditions may influence the microbial composition across gut regions are unknown. In this study, we used microsensors to measure in situ oxygen concentrations, pH, and redox potential along the length of the A. glabripennis larval gut from two North American populations. We then analyzed and compared bacterial and fungal gut communities of A. glabripennis within individual hosts along the length of the gut using 16S and ITS1 amplicon sequencing. The A. glabripennis midgut lumen was relatively anoxic (<0.01 kPa) with a pH gradient from 5.5 to 9, moving anterior to posterior. Redox potential was higher in the anterior midgut relative to posterior regions. No differences in physicochemistry were measured between the two populations of the beetle, but the two populations harbored different communities of bacteria and fungi. However, microbial composition of the A. glabripennis gut microbiota did not differ among gut regions despite physicochemical differences. Unlike other insect systems that have distinct gut compartmentalization and corresponding microbial assemblages, the A. glabripennis gut lacks dramatic morphological modifications, which may explain why discrete microbial community structures were not found along the digestive system.

Original languageEnglish (US)
Pages (from-to)27-35
Number of pages9
JournalJournal of Insect Physiology
Volume102
DOIs
StatePublished - Oct 1 2017

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Anoplophora glabripennis
fungal communities
bacterial communities
Insects
digestive system
Beetles
proctolin
Oxidation-Reduction
Population
Digestive System
Proton-Motive Force
Digestion
Fungi
Oxygen
Bacteria
Food
redox potential
midgut
insects
lignocellulose

All Science Journal Classification (ASJC) codes

  • Physiology
  • Insect Science

Cite this

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title = "Within gut physicochemical variation does not correspond to distinct resident fungal and bacterial communities in the tree-killing xylophage, Anoplophora glabripennis",
abstract = "Insect guts harbor diverse microbial assemblages that can be influenced by multiple factors, including gut physiology and interactions by the host with its environment. The Asian longhorned beetle (A. glabripennis; Cerambycidae: Lamiinae) is an invasive tree–killing insect that harbors a diverse consortium of fungal and bacterial gut associates that provision nutrients and facilitate lignocellulose digestion. The physicochemical conditions of the A. glabripennis gut and how these conditions may influence the microbial composition across gut regions are unknown. In this study, we used microsensors to measure in situ oxygen concentrations, pH, and redox potential along the length of the A. glabripennis larval gut from two North American populations. We then analyzed and compared bacterial and fungal gut communities of A. glabripennis within individual hosts along the length of the gut using 16S and ITS1 amplicon sequencing. The A. glabripennis midgut lumen was relatively anoxic (<0.01 kPa) with a pH gradient from 5.5 to 9, moving anterior to posterior. Redox potential was higher in the anterior midgut relative to posterior regions. No differences in physicochemistry were measured between the two populations of the beetle, but the two populations harbored different communities of bacteria and fungi. However, microbial composition of the A. glabripennis gut microbiota did not differ among gut regions despite physicochemical differences. Unlike other insect systems that have distinct gut compartmentalization and corresponding microbial assemblages, the A. glabripennis gut lacks dramatic morphological modifications, which may explain why discrete microbial community structures were not found along the digestive system.",
author = "Mason, {Charles J.} and Long, {David C.} and McCarthy, {Elizabeth M.} and Nivedita Nagachar and Cristina Rosa and Scully, {Erin D.} and Ming Tien and Kelli Hoover",
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Within gut physicochemical variation does not correspond to distinct resident fungal and bacterial communities in the tree-killing xylophage, Anoplophora glabripennis. / Mason, Charles J.; Long, David C.; McCarthy, Elizabeth M.; Nagachar, Nivedita; Rosa, Cristina; Scully, Erin D.; Tien, Ming; Hoover, Kelli.

In: Journal of Insect Physiology, Vol. 102, 01.10.2017, p. 27-35.

Research output: Contribution to journalArticle

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AU - Mason, Charles J.

AU - Long, David C.

AU - McCarthy, Elizabeth M.

AU - Nagachar, Nivedita

AU - Rosa, Cristina

AU - Scully, Erin D.

AU - Tien, Ming

AU - Hoover, Kelli

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