Wnt/β-catenin signaling regulates yes-associated protein (YAP) gene expression in colorectal carcinoma cells

Wesley M. Konsavage, Sydney L. Kyler, Sherri A. Rennoll, Ge Jin, Gregory Yochum

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Mutations in the Wnt/β-catenin pathway occur in most colorectal cancers (CRCs), and these mutations lead to increased nuclear accumulation of the β-catenin transcriptional co-activator. In the nucleus, β-catenin associates with TCF/LEF sequence specific transcription factors to activate target gene expression. The Hippo pathway restricts cellular growth by preventing nuclear accumulation of the Yes-associated protein (YAP) transcriptional co-activator. YAP expression is elevated in CRCs suggesting that, like Wnt/β-catenin signaling, the Hippo pathway may contribute to colorectal carcinogenesis. Regulation of YAP at the post-translational level has been well studied but the transcription factors that control YAP gene expression are unknown. Here we demonstrate that β-catenin/ TCF4 complexes bind a DNA enhancer element within the first intron of the YAP gene to drive YAP expression in CRC cells. As such, reducing β-catenin expression in CRC cells using shRNAs leads to decreased YAP mRNA and protein levels. YAP is abundantly expressed in the cytoplasm and nuclei of several established human colon cancer cell lines and this localization pattern is insensitive to plating density. Finally, we show that YAP expression is elevated in the majority of a panel of primary human colorectal tumors compared with its expression in uninvolved colonic mucosa, and that YAP and β-catenin localize to the nuclear compartment of tumor cells. Together, these results implicate YAP as an oncogene whose expression is driven by aberrant Wnt/β-catenin signaling in human CRC cells.

Original languageEnglish (US)
Pages (from-to)11730-11739
Number of pages10
JournalJournal of Biological Chemistry
Volume287
Issue number15
DOIs
StatePublished - Apr 6 2012

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Catenins
Gene expression
Colorectal Neoplasms
Cells
Gene Expression
Proteins
Tumors
Transcription Factors
Mutation
Wnt Signaling Pathway
Oncogene Proteins
Plating
Colonic Neoplasms
Introns
Carcinogenesis
Cytoplasm
Mucous Membrane
Cell Line

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Konsavage, Wesley M. ; Kyler, Sydney L. ; Rennoll, Sherri A. ; Jin, Ge ; Yochum, Gregory. / Wnt/β-catenin signaling regulates yes-associated protein (YAP) gene expression in colorectal carcinoma cells. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 15. pp. 11730-11739.
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Wnt/β-catenin signaling regulates yes-associated protein (YAP) gene expression in colorectal carcinoma cells. / Konsavage, Wesley M.; Kyler, Sydney L.; Rennoll, Sherri A.; Jin, Ge; Yochum, Gregory.

In: Journal of Biological Chemistry, Vol. 287, No. 15, 06.04.2012, p. 11730-11739.

Research output: Contribution to journalArticle

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