Wolbachia density and virulence attenuation after transfer into a novel host

E. A. McGraw, D. J. Merritt, J. N. Droller, S. L. O'Neill

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

The factors that control replication rate of the intracellular bacterium Wolbachia pipientis in its insect hosts are unknown and difficult to explore, given the complex interaction of symbiont and host genotypes. Using a strain of Wolbachia that is known to over-replicate and shorten the lifespan of its Drosophila melanogaster host, we have tracked the evolution of replication control in both somatic and reproductive tissues in a novel host/Wolbachia association. After transinfection (the transfer of a Wolbachia strain into a different species) of the over-replicating Wolbachia popcorn strain from D. melanogaster to Drosophila simulans, we demonstrated that initial high densities in the ovaries were in excess of what was required for perfect maternal transmission, and were likely causing reductions in reproductive fitness. Both densities and fitness costs associated with ovary infection rapidly declined in the generations after transinfection. The early death effect in D. simulans attenuated only slightly and was comparable to that induced in D. melanogaster. This study reveals a strong host involvement in Wolbachia replication rates, the independence of density control responses in different tissues, and the strength of natural selection acting on reproductive fitness.

Original languageEnglish (US)
Pages (from-to)2918-2923
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number5
DOIs
StatePublished - Mar 5 2002

Fingerprint

Wolbachia
Virulence
Drosophila melanogaster
Genetic Fitness
Ovary
Genetic Selection
Insects
Genotype
Mothers
Bacteria
Costs and Cost Analysis
Infection

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{9371d65e65df439db352ed886b9d6b19,
title = "Wolbachia density and virulence attenuation after transfer into a novel host",
abstract = "The factors that control replication rate of the intracellular bacterium Wolbachia pipientis in its insect hosts are unknown and difficult to explore, given the complex interaction of symbiont and host genotypes. Using a strain of Wolbachia that is known to over-replicate and shorten the lifespan of its Drosophila melanogaster host, we have tracked the evolution of replication control in both somatic and reproductive tissues in a novel host/Wolbachia association. After transinfection (the transfer of a Wolbachia strain into a different species) of the over-replicating Wolbachia popcorn strain from D. melanogaster to Drosophila simulans, we demonstrated that initial high densities in the ovaries were in excess of what was required for perfect maternal transmission, and were likely causing reductions in reproductive fitness. Both densities and fitness costs associated with ovary infection rapidly declined in the generations after transinfection. The early death effect in D. simulans attenuated only slightly and was comparable to that induced in D. melanogaster. This study reveals a strong host involvement in Wolbachia replication rates, the independence of density control responses in different tissues, and the strength of natural selection acting on reproductive fitness.",
author = "McGraw, {E. A.} and Merritt, {D. J.} and Droller, {J. N.} and O'Neill, {S. L.}",
year = "2002",
month = "3",
day = "5",
doi = "10.1073/pnas.052466499",
language = "English (US)",
volume = "99",
pages = "2918--2923",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "5",

}

Wolbachia density and virulence attenuation after transfer into a novel host. / McGraw, E. A.; Merritt, D. J.; Droller, J. N.; O'Neill, S. L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 5, 05.03.2002, p. 2918-2923.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Wolbachia density and virulence attenuation after transfer into a novel host

AU - McGraw, E. A.

AU - Merritt, D. J.

AU - Droller, J. N.

AU - O'Neill, S. L.

PY - 2002/3/5

Y1 - 2002/3/5

N2 - The factors that control replication rate of the intracellular bacterium Wolbachia pipientis in its insect hosts are unknown and difficult to explore, given the complex interaction of symbiont and host genotypes. Using a strain of Wolbachia that is known to over-replicate and shorten the lifespan of its Drosophila melanogaster host, we have tracked the evolution of replication control in both somatic and reproductive tissues in a novel host/Wolbachia association. After transinfection (the transfer of a Wolbachia strain into a different species) of the over-replicating Wolbachia popcorn strain from D. melanogaster to Drosophila simulans, we demonstrated that initial high densities in the ovaries were in excess of what was required for perfect maternal transmission, and were likely causing reductions in reproductive fitness. Both densities and fitness costs associated with ovary infection rapidly declined in the generations after transinfection. The early death effect in D. simulans attenuated only slightly and was comparable to that induced in D. melanogaster. This study reveals a strong host involvement in Wolbachia replication rates, the independence of density control responses in different tissues, and the strength of natural selection acting on reproductive fitness.

AB - The factors that control replication rate of the intracellular bacterium Wolbachia pipientis in its insect hosts are unknown and difficult to explore, given the complex interaction of symbiont and host genotypes. Using a strain of Wolbachia that is known to over-replicate and shorten the lifespan of its Drosophila melanogaster host, we have tracked the evolution of replication control in both somatic and reproductive tissues in a novel host/Wolbachia association. After transinfection (the transfer of a Wolbachia strain into a different species) of the over-replicating Wolbachia popcorn strain from D. melanogaster to Drosophila simulans, we demonstrated that initial high densities in the ovaries were in excess of what was required for perfect maternal transmission, and were likely causing reductions in reproductive fitness. Both densities and fitness costs associated with ovary infection rapidly declined in the generations after transinfection. The early death effect in D. simulans attenuated only slightly and was comparable to that induced in D. melanogaster. This study reveals a strong host involvement in Wolbachia replication rates, the independence of density control responses in different tissues, and the strength of natural selection acting on reproductive fitness.

UR - http://www.scopus.com/inward/record.url?scp=0037022635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037022635&partnerID=8YFLogxK

U2 - 10.1073/pnas.052466499

DO - 10.1073/pnas.052466499

M3 - Article

C2 - 11880639

AN - SCOPUS:0037022635

VL - 99

SP - 2918

EP - 2923

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 5

ER -