Wood chemistry analysis and expression profiling of a poplar clone expressing a tyrosine-rich peptide

Yi Xu, Chin Fu Chen, Tina P. Thomas, Parastoo Azadi, Brett Diehl, Chung Jui Tsai, Nicole Brown, John Edward Carlson, Ming Tien, Haiying Liang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Key message; Our study has identified pathways and gene candidates that may be associated with the greater flexibility and digestibility of the poplar cell walls. With the goal of facilitating lignin removal during the utilization of woody biomass as a biofuel feedstock, we previously transformed a hybrid poplar clone with a partial cDNA sequence encoding a tyrosine- and hydroxyproline-rich glycoprotein from parsley. A number of the transgenic lines released more polysaccharides following protease digestion and were more flexible than wild-type plants, but otherwise normal in phenotype. Here, we report that overexpression of the tyrosine-rich peptide encoding sequence in these transgenic poplar plants did not significantly alter total lignin quantity or quality (S/G lignin ratio), five- and six-carbon sugar contents, growth rate, or susceptibility to a major poplar fungal pathogen, Septoria musiva. Whole-genome microarray analysis revealed a total of 411 differentially expressed transcripts in transgenic lines, all with decreased transcript abundance relative to wild-type plants. Their corresponding genes were overrepresented in functional categories such as secondary metabolism, amino acid metabolism, and energy metabolism. Transcript abundance was decreased primarily for five types of genes encoding proteins involved in cell-wall organization and in lignin biosynthesis. The expression of a subset of 19 of the differentially regulated genes by qRT-PCR validated the microarray results. Our study has identified pathways and gene candidates that may be the underlying cause for the enhanced flexibility and digestibility of the stems of poplar plants expressing the TYR transgene.

Original languageEnglish (US)
Pages (from-to)1827-1841
Number of pages15
JournalPlant Cell Reports
Volume32
Issue number12
DOIs
StatePublished - Dec 1 2013

Fingerprint

wood chemistry
tyrosine
peptides
clones
lignin
genetically modified organisms
genes
digestibility
cell walls
Septoria
parsley
carbon
hydroxyproline
amino acid metabolism
feedstocks
biofuels
sugar content
energy metabolism
transgenes
glycoproteins

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Xu, Y., Chen, C. F., Thomas, T. P., Azadi, P., Diehl, B., Tsai, C. J., ... Liang, H. (2013). Wood chemistry analysis and expression profiling of a poplar clone expressing a tyrosine-rich peptide. Plant Cell Reports, 32(12), 1827-1841. https://doi.org/10.1007/s00299-013-1496-0
Xu, Yi ; Chen, Chin Fu ; Thomas, Tina P. ; Azadi, Parastoo ; Diehl, Brett ; Tsai, Chung Jui ; Brown, Nicole ; Carlson, John Edward ; Tien, Ming ; Liang, Haiying. / Wood chemistry analysis and expression profiling of a poplar clone expressing a tyrosine-rich peptide. In: Plant Cell Reports. 2013 ; Vol. 32, No. 12. pp. 1827-1841.
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Wood chemistry analysis and expression profiling of a poplar clone expressing a tyrosine-rich peptide. / Xu, Yi; Chen, Chin Fu; Thomas, Tina P.; Azadi, Parastoo; Diehl, Brett; Tsai, Chung Jui; Brown, Nicole; Carlson, John Edward; Tien, Ming; Liang, Haiying.

In: Plant Cell Reports, Vol. 32, No. 12, 01.12.2013, p. 1827-1841.

Research output: Contribution to journalArticle

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