X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway

Katsuhiko S. Murakami, Yeonoh Shin, Charles L. Turnbough, Vadim Molodtsov

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Reiterative transcription is a noncanonical form of RNA synthesis in which a nucleotide specified by a single base in the DNA template is repetitively added to the nascent transcript. Here we determined the crystal structure of an RNA polymerase, the bacterial enzyme from Thermus thermophilus, engaged in reiterative transcription during transcription initiation at a promoter resembling the pyrG promoter of Bacillus subtilis. The structure reveals that the reiterative transcript detours from the dedicated RNA exit channel and extends toward the main channel of the enzyme, thereby allowing RNA extension without displacement of the promoter recognition σ-factor. Nascent transcripts containing reiteratively added G residues are eventually extended by nonreiterative transcription, revealing an atypical pathway for the formation of a transcription elongation complex.

Original languageEnglish (US)
Pages (from-to)8211-8216
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number31
DOIs
StatePublished - Aug 1 2017

All Science Journal Classification (ASJC) codes

  • General

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