XY sex reversal in the wood lemming is associated with deletion of Xp21-23, revealed by chromosome microdissection and fluorescence in situ hybridization

Wansheng Liu, Leif Eriksson, Karl Fredga

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In the wood lemming (Myopus schisticolor), XY sex reversal occurs naturally because of the presence of an X chromosome variant designated X(*). The two types of X chromosome, X and X(*), can be distinguished by G-banding, and analyses have demonstrated complex rearrangements of the short arm of X(*). Here, chromosomal microdissection, degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and fluorescence in site hybridization (FISH) techniques have been used to generate and map DNA probes for different parts of the X and X(*) chromosomes. The results showed that the region of Xp21-23 is deleted from the X(*) and some of the deleted DNA sequences are homologous to the mouse gamma-satellite. The deletion must be associated with the sex reversal in this species. FISH experiments with dissected probes of X and distal half of Xq provided evidence for presence of homologous sequences between large regions of the X and Y chromosomes, including euchromatic and heterochromatic parts of the sex chromosomes. The findings of this study will be of significance for further cloning of important candidate gene(s) responsible for the XY sex reversal.

Original languageEnglish (US)
Pages (from-to)379-383
Number of pages5
JournalChromosome Research
Volume6
Issue number5
DOIs
StatePublished - Nov 26 1998

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Microdissection
Arvicolinae
X Chromosome
Fluorescence In Situ Hybridization
Chromosomes
Fluorescence
Safe Sex
Sex Chromosomes
Y Chromosome
DNA Probes
Sequence Homology
Organism Cloning
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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title = "XY sex reversal in the wood lemming is associated with deletion of Xp21-23, revealed by chromosome microdissection and fluorescence in situ hybridization",
abstract = "In the wood lemming (Myopus schisticolor), XY sex reversal occurs naturally because of the presence of an X chromosome variant designated X(*). The two types of X chromosome, X and X(*), can be distinguished by G-banding, and analyses have demonstrated complex rearrangements of the short arm of X(*). Here, chromosomal microdissection, degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and fluorescence in site hybridization (FISH) techniques have been used to generate and map DNA probes for different parts of the X and X(*) chromosomes. The results showed that the region of Xp21-23 is deleted from the X(*) and some of the deleted DNA sequences are homologous to the mouse gamma-satellite. The deletion must be associated with the sex reversal in this species. FISH experiments with dissected probes of X and distal half of Xq provided evidence for presence of homologous sequences between large regions of the X and Y chromosomes, including euchromatic and heterochromatic parts of the sex chromosomes. The findings of this study will be of significance for further cloning of important candidate gene(s) responsible for the XY sex reversal.",
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XY sex reversal in the wood lemming is associated with deletion of Xp21-23, revealed by chromosome microdissection and fluorescence in situ hybridization. / Liu, Wansheng; Eriksson, Leif; Fredga, Karl.

In: Chromosome Research, Vol. 6, No. 5, 26.11.1998, p. 379-383.

Research output: Contribution to journalArticle

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