Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2

Moshe Herzberg, Andrés F. González Barrios, Youngsoon Um, William E. Bentley, Ian K. Kaye, Thomas Keith Wood

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The putative transport protein YdgG (344 aa) was induced 5-fold in Escherichia coli JM109 biofilms (Appl. Microbiol. Biotechnol. 64:515, 2004) and was confirmed here to control biofilm formation in complex and minimal media since deleting ydgG increased biofilm formation by 4- and 2-fold in complex and minimal media supplemented with glucose using a 96-well microtitre assay as well as increased biomass by 600-fold in flow chambers with minimal media supplemented with glucose. YdgG was also found to negatively modulate motility since transcription of flagella genes (qseB, flhD, fliA, and fliC) and a motility gene (motA) increased by 15- to 120-fold upon deletion of ydgG. Deletion of ydgG also decreased extracellular activity of autoinducer-2 by 4- to 13-fold and increased intracellular activity when glucose was added to the media by 10- to 17-fold. Using DNA microarrays and by comparing the biofilm mutant vs. the wild-type, we found deleting ydgG caused 31% of the bacterial chromosome to be differentially induced more than 2-fold, and 7.6% of the genes were repressed by more than 2-fold. YdgG not only negatively modulates expression of flagella- and motility-related genes but also all the other known products essential for biofilm formation: 4 known operons for flagella synthesis and motility (flgABCDEFGHIJ,fliEFGHIJK,fliLMNOPQR, and motABcheAW), adhesion determinants (type 1 fimbriae and the autotransporter protein Ag43), curli production, colanic acid production, and production of β-1,6-N-acetyl-D- glucoseamine polysaccharide adhesin. In addition, acrEF was induced 24-fold and this locus encodes for a known transporter of indole which is a stationary-phase, extracellular signal; indole has also been shown to influence biofilm formation. Through the microarrays, new genes related to biofilm formation were identified including transport proteins (yihN and yihP), polysialic acid production (gutM and gutQ), phage (yjfR and alpA), methionine biosynthesis (metR), biotin and thiamine biosynthesis (bioF and thiDFH), anaerobic metabolim (focB, hyfACDR, ttdA, and fumB), and genes with unknown function (ybfG, yceO, yjhQ, and yjbE). Hence, it appears ydgG controls the secretion of the quorum sensing signal AI-2 and so has been renamed sqsA.

Original languageEnglish (US)
Title of host publication05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings
Pages8784
Number of pages1
StatePublished - 2005
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

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Biofilms
Escherichia coli
Genes
Glucose
Biosynthesis
Microarrays
Bacteriophages
Acids
Transcription
Polysaccharides
Chromosomes
Assays
Biomass
DNA
Adhesion
Proteins

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Herzberg, M., González Barrios, A. F., Um, Y., Bentley, W. E., Kaye, I. K., & Wood, T. K. (2005). Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2. In 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings (pp. 8784)
Herzberg, Moshe ; González Barrios, Andrés F. ; Um, Youngsoon ; Bentley, William E. ; Kaye, Ian K. ; Wood, Thomas Keith. / Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2. 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings. 2005. pp. 8784
@inproceedings{52dba2d0ada04207a65af032b72cf04d,
title = "Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2",
abstract = "The putative transport protein YdgG (344 aa) was induced 5-fold in Escherichia coli JM109 biofilms (Appl. Microbiol. Biotechnol. 64:515, 2004) and was confirmed here to control biofilm formation in complex and minimal media since deleting ydgG increased biofilm formation by 4- and 2-fold in complex and minimal media supplemented with glucose using a 96-well microtitre assay as well as increased biomass by 600-fold in flow chambers with minimal media supplemented with glucose. YdgG was also found to negatively modulate motility since transcription of flagella genes (qseB, flhD, fliA, and fliC) and a motility gene (motA) increased by 15- to 120-fold upon deletion of ydgG. Deletion of ydgG also decreased extracellular activity of autoinducer-2 by 4- to 13-fold and increased intracellular activity when glucose was added to the media by 10- to 17-fold. Using DNA microarrays and by comparing the biofilm mutant vs. the wild-type, we found deleting ydgG caused 31{\%} of the bacterial chromosome to be differentially induced more than 2-fold, and 7.6{\%} of the genes were repressed by more than 2-fold. YdgG not only negatively modulates expression of flagella- and motility-related genes but also all the other known products essential for biofilm formation: 4 known operons for flagella synthesis and motility (flgABCDEFGHIJ,fliEFGHIJK,fliLMNOPQR, and motABcheAW), adhesion determinants (type 1 fimbriae and the autotransporter protein Ag43), curli production, colanic acid production, and production of β-1,6-N-acetyl-D- glucoseamine polysaccharide adhesin. In addition, acrEF was induced 24-fold and this locus encodes for a known transporter of indole which is a stationary-phase, extracellular signal; indole has also been shown to influence biofilm formation. Through the microarrays, new genes related to biofilm formation were identified including transport proteins (yihN and yihP), polysialic acid production (gutM and gutQ), phage (yjfR and alpA), methionine biosynthesis (metR), biotin and thiamine biosynthesis (bioF and thiDFH), anaerobic metabolim (focB, hyfACDR, ttdA, and fumB), and genes with unknown function (ybfG, yceO, yjhQ, and yjbE). Hence, it appears ydgG controls the secretion of the quorum sensing signal AI-2 and so has been renamed sqsA.",
author = "Moshe Herzberg and {Gonz{\'a}lez Barrios}, {Andr{\'e}s F.} and Youngsoon Um and Bentley, {William E.} and Kaye, {Ian K.} and Wood, {Thomas Keith}",
year = "2005",
language = "English (US)",
pages = "8784",
booktitle = "05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings",

}

Herzberg, M, González Barrios, AF, Um, Y, Bentley, WE, Kaye, IK & Wood, TK 2005, Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2. in 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings. pp. 8784, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 10/30/05.

Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2. / Herzberg, Moshe; González Barrios, Andrés F.; Um, Youngsoon; Bentley, William E.; Kaye, Ian K.; Wood, Thomas Keith.

05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings. 2005. p. 8784.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2

AU - Herzberg, Moshe

AU - González Barrios, Andrés F.

AU - Um, Youngsoon

AU - Bentley, William E.

AU - Kaye, Ian K.

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N2 - The putative transport protein YdgG (344 aa) was induced 5-fold in Escherichia coli JM109 biofilms (Appl. Microbiol. Biotechnol. 64:515, 2004) and was confirmed here to control biofilm formation in complex and minimal media since deleting ydgG increased biofilm formation by 4- and 2-fold in complex and minimal media supplemented with glucose using a 96-well microtitre assay as well as increased biomass by 600-fold in flow chambers with minimal media supplemented with glucose. YdgG was also found to negatively modulate motility since transcription of flagella genes (qseB, flhD, fliA, and fliC) and a motility gene (motA) increased by 15- to 120-fold upon deletion of ydgG. Deletion of ydgG also decreased extracellular activity of autoinducer-2 by 4- to 13-fold and increased intracellular activity when glucose was added to the media by 10- to 17-fold. Using DNA microarrays and by comparing the biofilm mutant vs. the wild-type, we found deleting ydgG caused 31% of the bacterial chromosome to be differentially induced more than 2-fold, and 7.6% of the genes were repressed by more than 2-fold. YdgG not only negatively modulates expression of flagella- and motility-related genes but also all the other known products essential for biofilm formation: 4 known operons for flagella synthesis and motility (flgABCDEFGHIJ,fliEFGHIJK,fliLMNOPQR, and motABcheAW), adhesion determinants (type 1 fimbriae and the autotransporter protein Ag43), curli production, colanic acid production, and production of β-1,6-N-acetyl-D- glucoseamine polysaccharide adhesin. In addition, acrEF was induced 24-fold and this locus encodes for a known transporter of indole which is a stationary-phase, extracellular signal; indole has also been shown to influence biofilm formation. Through the microarrays, new genes related to biofilm formation were identified including transport proteins (yihN and yihP), polysialic acid production (gutM and gutQ), phage (yjfR and alpA), methionine biosynthesis (metR), biotin and thiamine biosynthesis (bioF and thiDFH), anaerobic metabolim (focB, hyfACDR, ttdA, and fumB), and genes with unknown function (ybfG, yceO, yjhQ, and yjbE). Hence, it appears ydgG controls the secretion of the quorum sensing signal AI-2 and so has been renamed sqsA.

AB - The putative transport protein YdgG (344 aa) was induced 5-fold in Escherichia coli JM109 biofilms (Appl. Microbiol. Biotechnol. 64:515, 2004) and was confirmed here to control biofilm formation in complex and minimal media since deleting ydgG increased biofilm formation by 4- and 2-fold in complex and minimal media supplemented with glucose using a 96-well microtitre assay as well as increased biomass by 600-fold in flow chambers with minimal media supplemented with glucose. YdgG was also found to negatively modulate motility since transcription of flagella genes (qseB, flhD, fliA, and fliC) and a motility gene (motA) increased by 15- to 120-fold upon deletion of ydgG. Deletion of ydgG also decreased extracellular activity of autoinducer-2 by 4- to 13-fold and increased intracellular activity when glucose was added to the media by 10- to 17-fold. Using DNA microarrays and by comparing the biofilm mutant vs. the wild-type, we found deleting ydgG caused 31% of the bacterial chromosome to be differentially induced more than 2-fold, and 7.6% of the genes were repressed by more than 2-fold. YdgG not only negatively modulates expression of flagella- and motility-related genes but also all the other known products essential for biofilm formation: 4 known operons for flagella synthesis and motility (flgABCDEFGHIJ,fliEFGHIJK,fliLMNOPQR, and motABcheAW), adhesion determinants (type 1 fimbriae and the autotransporter protein Ag43), curli production, colanic acid production, and production of β-1,6-N-acetyl-D- glucoseamine polysaccharide adhesin. In addition, acrEF was induced 24-fold and this locus encodes for a known transporter of indole which is a stationary-phase, extracellular signal; indole has also been shown to influence biofilm formation. Through the microarrays, new genes related to biofilm formation were identified including transport proteins (yihN and yihP), polysialic acid production (gutM and gutQ), phage (yjfR and alpA), methionine biosynthesis (metR), biotin and thiamine biosynthesis (bioF and thiDFH), anaerobic metabolim (focB, hyfACDR, ttdA, and fumB), and genes with unknown function (ybfG, yceO, yjhQ, and yjbE). Hence, it appears ydgG controls the secretion of the quorum sensing signal AI-2 and so has been renamed sqsA.

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Herzberg M, González Barrios AF, Um Y, Bentley WE, Kaye IK, Wood TK. Ydgg (renamed Sqsa) controls biofilm formation in Escherichia coli K12 by altering secretion of the quorum-sensing signal autoinducer-2. In 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings. 2005. p. 8784