Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation

Lynne M. Apone, Ching Man A. Virbasius, Joseph C. Reese, Michael R. Green

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA-box binding protein and associated factors (TAF(II)s). In vitro experiments have suggested that TAF(II)s are essential coactivators required for RNA polymerase II-directed transcription activation. Here, for the first time, we analyze systematically the in vivo function of a specific TAF(II), yeast TAF(II)90 (yTAF(II)90). We show that functional inactivation of yTAF(II)90 by temperature-sensitive mutations or depletion leads to arrest at the G2/M phase of the cell cycle. Unexpectedly, in the absence of functional yTAF(II)90, a variety of endogenous yeast genes were all transcribed normally, including those driven by well-characterized activators. Taken together, our results indicate that yTAF(II)90 is not required for transcription activation in general, and reveal linkages between TAF(II) function and cell-cycle progression.

Original languageEnglish (US)
Pages (from-to)2368-2380
Number of pages13
JournalGenes and Development
Volume10
Issue number18
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'Yeast TAF(II)90 is required for cell-cycle progression through G<sub>2</sub>/M but not for general transcription activation'. Together they form a unique fingerprint.

Cite this