Yeasts associated with plums and their potential for controlling brown rot after harvest

Wojciech J. Janisiewicz, Wayne M. Jurick, Kari Anne Peter, Cletus P. Kurtzman, Jeffrey S. Buyer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Bacterial and yeast antagonists isolated from fruit surfaces have been effective in controlling various post-harvest diseases, and several microbial antagonists have been developed into commercial products. Our knowledge of the fruit microbial community, with the exception of grapes, apples and some citrus fruit, is rudimentary and the potential of the resident yeasts for biocontrol remains largely unknown. We determined the occurrence of yeasts on plum surfaces during fruit development from the pre-hardening stage until harvest for 2 years. A total of 16 species from 13 genera were isolated. Species from three genera, basidiomycetes Rhodotorula (29.5%) and Sporidiobolus (24.7%) and the dimorphic ascomycete genus Aureobasidium (24.7%), constituted 78.7% of all isolations and were recovered throughout fruit development, while Cryptococcus spp. constituted only 6.2% of the total plum isolates. The yeast community in the final sampling was significantly different from the first three samplings, reflecting a rapidly changing fruit habitat during the maturation of fruit. For example, Hanseniaspora, Pichia, Zygosaccharomyces and Wickerhamomyces occurred only on the most mature fruit. Screening of the yeasts for antagonistic activity against Monilinia fructicola, a fungus that causes brown rot, revealed a range of biocontrol activities. Several isolates provided complete control of the decay on plums, challenged with a pathogen suspension of 103 conidia/ml and > 90% of control on fruit inoculated with the pathogen at a concentration 10 times higher. Some of the best antagonists included A. pullulans and R. phylloplana. Populations of both of these antagonists increased rapidly by several orders of magnitude in wounds of plums incubated at 24oC and 4oC. Our results indicate that plum surfaces harbour several yeast species, with excellent potential for use in biological control of brown rot of stone fruits. Published 2014. This article is a U.S. Government work and is in the public domain in the USA

Original languageEnglish (US)
Pages (from-to)207-218
Number of pages12
JournalYeast
Volume31
Issue number6
DOIs
StatePublished - Jan 1 2014

Fingerprint

Fruits
Yeast
Fruit
Yeasts
Biocontrol
Pathogens
Hanseniaspora
Citrus fruits
Zygosaccharomyces
Sampling
Rhodotorula
Prunus domestica
Cryptococcus
Basidiomycota
Ascomycota
Fungal Spores
Pichia
Citrus
Public Sector
Vitis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Janisiewicz, W. J., Jurick, W. M., Peter, K. A., Kurtzman, C. P., & Buyer, J. S. (2014). Yeasts associated with plums and their potential for controlling brown rot after harvest. Yeast, 31(6), 207-218. https://doi.org/10.1002/yea.3009
Janisiewicz, Wojciech J. ; Jurick, Wayne M. ; Peter, Kari Anne ; Kurtzman, Cletus P. ; Buyer, Jeffrey S. / Yeasts associated with plums and their potential for controlling brown rot after harvest. In: Yeast. 2014 ; Vol. 31, No. 6. pp. 207-218.
@article{d82d362de1304a8097fd66308e6c351d,
title = "Yeasts associated with plums and their potential for controlling brown rot after harvest",
abstract = "Bacterial and yeast antagonists isolated from fruit surfaces have been effective in controlling various post-harvest diseases, and several microbial antagonists have been developed into commercial products. Our knowledge of the fruit microbial community, with the exception of grapes, apples and some citrus fruit, is rudimentary and the potential of the resident yeasts for biocontrol remains largely unknown. We determined the occurrence of yeasts on plum surfaces during fruit development from the pre-hardening stage until harvest for 2 years. A total of 16 species from 13 genera were isolated. Species from three genera, basidiomycetes Rhodotorula (29.5{\%}) and Sporidiobolus (24.7{\%}) and the dimorphic ascomycete genus Aureobasidium (24.7{\%}), constituted 78.7{\%} of all isolations and were recovered throughout fruit development, while Cryptococcus spp. constituted only 6.2{\%} of the total plum isolates. The yeast community in the final sampling was significantly different from the first three samplings, reflecting a rapidly changing fruit habitat during the maturation of fruit. For example, Hanseniaspora, Pichia, Zygosaccharomyces and Wickerhamomyces occurred only on the most mature fruit. Screening of the yeasts for antagonistic activity against Monilinia fructicola, a fungus that causes brown rot, revealed a range of biocontrol activities. Several isolates provided complete control of the decay on plums, challenged with a pathogen suspension of 103 conidia/ml and > 90{\%} of control on fruit inoculated with the pathogen at a concentration 10 times higher. Some of the best antagonists included A. pullulans and R. phylloplana. Populations of both of these antagonists increased rapidly by several orders of magnitude in wounds of plums incubated at 24oC and 4oC. Our results indicate that plum surfaces harbour several yeast species, with excellent potential for use in biological control of brown rot of stone fruits. Published 2014. This article is a U.S. Government work and is in the public domain in the USA",
author = "Janisiewicz, {Wojciech J.} and Jurick, {Wayne M.} and Peter, {Kari Anne} and Kurtzman, {Cletus P.} and Buyer, {Jeffrey S.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1002/yea.3009",
language = "English (US)",
volume = "31",
pages = "207--218",
journal = "Yeast",
issn = "0749-503X",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

Janisiewicz, WJ, Jurick, WM, Peter, KA, Kurtzman, CP & Buyer, JS 2014, 'Yeasts associated with plums and their potential for controlling brown rot after harvest', Yeast, vol. 31, no. 6, pp. 207-218. https://doi.org/10.1002/yea.3009

Yeasts associated with plums and their potential for controlling brown rot after harvest. / Janisiewicz, Wojciech J.; Jurick, Wayne M.; Peter, Kari Anne; Kurtzman, Cletus P.; Buyer, Jeffrey S.

In: Yeast, Vol. 31, No. 6, 01.01.2014, p. 207-218.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Yeasts associated with plums and their potential for controlling brown rot after harvest

AU - Janisiewicz, Wojciech J.

AU - Jurick, Wayne M.

AU - Peter, Kari Anne

AU - Kurtzman, Cletus P.

AU - Buyer, Jeffrey S.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Bacterial and yeast antagonists isolated from fruit surfaces have been effective in controlling various post-harvest diseases, and several microbial antagonists have been developed into commercial products. Our knowledge of the fruit microbial community, with the exception of grapes, apples and some citrus fruit, is rudimentary and the potential of the resident yeasts for biocontrol remains largely unknown. We determined the occurrence of yeasts on plum surfaces during fruit development from the pre-hardening stage until harvest for 2 years. A total of 16 species from 13 genera were isolated. Species from three genera, basidiomycetes Rhodotorula (29.5%) and Sporidiobolus (24.7%) and the dimorphic ascomycete genus Aureobasidium (24.7%), constituted 78.7% of all isolations and were recovered throughout fruit development, while Cryptococcus spp. constituted only 6.2% of the total plum isolates. The yeast community in the final sampling was significantly different from the first three samplings, reflecting a rapidly changing fruit habitat during the maturation of fruit. For example, Hanseniaspora, Pichia, Zygosaccharomyces and Wickerhamomyces occurred only on the most mature fruit. Screening of the yeasts for antagonistic activity against Monilinia fructicola, a fungus that causes brown rot, revealed a range of biocontrol activities. Several isolates provided complete control of the decay on plums, challenged with a pathogen suspension of 103 conidia/ml and > 90% of control on fruit inoculated with the pathogen at a concentration 10 times higher. Some of the best antagonists included A. pullulans and R. phylloplana. Populations of both of these antagonists increased rapidly by several orders of magnitude in wounds of plums incubated at 24oC and 4oC. Our results indicate that plum surfaces harbour several yeast species, with excellent potential for use in biological control of brown rot of stone fruits. Published 2014. This article is a U.S. Government work and is in the public domain in the USA

AB - Bacterial and yeast antagonists isolated from fruit surfaces have been effective in controlling various post-harvest diseases, and several microbial antagonists have been developed into commercial products. Our knowledge of the fruit microbial community, with the exception of grapes, apples and some citrus fruit, is rudimentary and the potential of the resident yeasts for biocontrol remains largely unknown. We determined the occurrence of yeasts on plum surfaces during fruit development from the pre-hardening stage until harvest for 2 years. A total of 16 species from 13 genera were isolated. Species from three genera, basidiomycetes Rhodotorula (29.5%) and Sporidiobolus (24.7%) and the dimorphic ascomycete genus Aureobasidium (24.7%), constituted 78.7% of all isolations and were recovered throughout fruit development, while Cryptococcus spp. constituted only 6.2% of the total plum isolates. The yeast community in the final sampling was significantly different from the first three samplings, reflecting a rapidly changing fruit habitat during the maturation of fruit. For example, Hanseniaspora, Pichia, Zygosaccharomyces and Wickerhamomyces occurred only on the most mature fruit. Screening of the yeasts for antagonistic activity against Monilinia fructicola, a fungus that causes brown rot, revealed a range of biocontrol activities. Several isolates provided complete control of the decay on plums, challenged with a pathogen suspension of 103 conidia/ml and > 90% of control on fruit inoculated with the pathogen at a concentration 10 times higher. Some of the best antagonists included A. pullulans and R. phylloplana. Populations of both of these antagonists increased rapidly by several orders of magnitude in wounds of plums incubated at 24oC and 4oC. Our results indicate that plum surfaces harbour several yeast species, with excellent potential for use in biological control of brown rot of stone fruits. Published 2014. This article is a U.S. Government work and is in the public domain in the USA

UR - http://www.scopus.com/inward/record.url?scp=84902006357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902006357&partnerID=8YFLogxK

U2 - 10.1002/yea.3009

DO - 10.1002/yea.3009

M3 - Article

C2 - 24687564

AN - SCOPUS:84902006357

VL - 31

SP - 207

EP - 218

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 6

ER -