Abstract
Background and aim: Some degenerative diseases of the nervous system have been linked to hormonal imbalance in postmenopausal women. It is argued that young coconut juice (YCJ) could have some estrogen-like characteristics, but this is still debatable. Our aim was to investigate this argument, and to examine whether YCJ has any neuroprotective effects. Materials and methods: Four groups of female rats (10 in each group) were included in this study. These included sham-operated, overiectomized (ovx), ovx and receiving estradiol benzoate (EB) injections intraperitoneally, and ovx and receiving YCJ orally. At the end of the five-week study, the rats were sacrificed, and their serum estradiol (E2) level was measured by chemiluminescent immunoassay. Moreover, the rat brains were excised, and the cortical pyramidal neurons were examined using markers of neuronal cell death, namely anti-neurofilament (NF200) and antiparvalbumin (PV) antibodies. Results: Our results showed that the rat group which received YCJ had its serum E2 level significantly (P<0.05) higher than the ovx group which did not receive any treatment, and the sham-operated group. A similar trend was observed with the group which received EB injections, but no significant difference was present when the latter was compared with the shamoperated group. In addition, a significant reduction in neuronal cell death was observed in the YCJ-treated group, as compared to the ovx group which did not receive any treatment. This was indicated by the significantly (P<0.05) higher number of neurons which were immunopositive for NF200 and PV. Interestingly, the number of these neurons was also significantly (P<0.05) higher in the YCJ group, as compared to the EB group. Conclusion: This study confirms the argument that YCJ has estrogen-like characteristics, and it also adds more evidence to the observation that hormonal imbalance could induce some brain pathologies in females.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 667-674 |
| Number of pages | 8 |
| Journal | Histology and Histopathology |
| Volume | 24 |
| Issue number | 6 |
| State | Published - Jun 1 2009 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Histology
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Young coconut juice significantly reduces histopathological changes in the brain that is induced by hormonal imbalance : A possible implication to postmenopausal women. / Radenahmad, Nisaudah; Saleh, Farid; Sawangjaroen, Kitja; Rundorn, Wilart; Withyachumnarnkul, Boornsirm; Connor, James.
In: Histology and Histopathology, Vol. 24, No. 6, 01.06.2009, p. 667-674.Research output: Contribution to journal › Article
TY - JOUR
T1 - Young coconut juice significantly reduces histopathological changes in the brain that is induced by hormonal imbalance
T2 - A possible implication to postmenopausal women
AU - Radenahmad, Nisaudah
AU - Saleh, Farid
AU - Sawangjaroen, Kitja
AU - Rundorn, Wilart
AU - Withyachumnarnkul, Boornsirm
AU - Connor, James
PY - 2009/6/1
Y1 - 2009/6/1
N2 - Background and aim: Some degenerative diseases of the nervous system have been linked to hormonal imbalance in postmenopausal women. It is argued that young coconut juice (YCJ) could have some estrogen-like characteristics, but this is still debatable. Our aim was to investigate this argument, and to examine whether YCJ has any neuroprotective effects. Materials and methods: Four groups of female rats (10 in each group) were included in this study. These included sham-operated, overiectomized (ovx), ovx and receiving estradiol benzoate (EB) injections intraperitoneally, and ovx and receiving YCJ orally. At the end of the five-week study, the rats were sacrificed, and their serum estradiol (E2) level was measured by chemiluminescent immunoassay. Moreover, the rat brains were excised, and the cortical pyramidal neurons were examined using markers of neuronal cell death, namely anti-neurofilament (NF200) and antiparvalbumin (PV) antibodies. Results: Our results showed that the rat group which received YCJ had its serum E2 level significantly (P<0.05) higher than the ovx group which did not receive any treatment, and the sham-operated group. A similar trend was observed with the group which received EB injections, but no significant difference was present when the latter was compared with the shamoperated group. In addition, a significant reduction in neuronal cell death was observed in the YCJ-treated group, as compared to the ovx group which did not receive any treatment. This was indicated by the significantly (P<0.05) higher number of neurons which were immunopositive for NF200 and PV. Interestingly, the number of these neurons was also significantly (P<0.05) higher in the YCJ group, as compared to the EB group. Conclusion: This study confirms the argument that YCJ has estrogen-like characteristics, and it also adds more evidence to the observation that hormonal imbalance could induce some brain pathologies in females.
AB - Background and aim: Some degenerative diseases of the nervous system have been linked to hormonal imbalance in postmenopausal women. It is argued that young coconut juice (YCJ) could have some estrogen-like characteristics, but this is still debatable. Our aim was to investigate this argument, and to examine whether YCJ has any neuroprotective effects. Materials and methods: Four groups of female rats (10 in each group) were included in this study. These included sham-operated, overiectomized (ovx), ovx and receiving estradiol benzoate (EB) injections intraperitoneally, and ovx and receiving YCJ orally. At the end of the five-week study, the rats were sacrificed, and their serum estradiol (E2) level was measured by chemiluminescent immunoassay. Moreover, the rat brains were excised, and the cortical pyramidal neurons were examined using markers of neuronal cell death, namely anti-neurofilament (NF200) and antiparvalbumin (PV) antibodies. Results: Our results showed that the rat group which received YCJ had its serum E2 level significantly (P<0.05) higher than the ovx group which did not receive any treatment, and the sham-operated group. A similar trend was observed with the group which received EB injections, but no significant difference was present when the latter was compared with the shamoperated group. In addition, a significant reduction in neuronal cell death was observed in the YCJ-treated group, as compared to the ovx group which did not receive any treatment. This was indicated by the significantly (P<0.05) higher number of neurons which were immunopositive for NF200 and PV. Interestingly, the number of these neurons was also significantly (P<0.05) higher in the YCJ group, as compared to the EB group. Conclusion: This study confirms the argument that YCJ has estrogen-like characteristics, and it also adds more evidence to the observation that hormonal imbalance could induce some brain pathologies in females.
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M3 - Article
C2 - 19337965
AN - SCOPUS:67650246234
VL - 24
SP - 667
EP - 674
JO - Histology and Histopathology
JF - Histology and Histopathology
SN - 0213-3911
IS - 6
ER -