YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice

Satabdi Nandi, Guanxiang Liang, Vishal Sindhava, Rajesh Angireddy, Arindam Basu, Sarmistha Banerjee, Suchita Hodawadekar, Yue Zhang, Narayan G. Avadhani, Ranjan Sen, Michael L. Atchison

Research output: Contribution to journalArticle

Abstract

Immunoglobulin class switch recombination (CSR) occurs in activated B cells with increased mitochondrial mass and membrane potential. Transcription factor Yin Yang 1 (YY1) is critical for CSR and for formation of the DNA loops involved in this process. We therefore sought to determine if YY1 knockout impacts mitochondrial gene expression and mitochondrial function in murine splenic B cells, providing a potential mechanism for regulating CSR. We identified numerous genes in splenic B cells differentially regulated when cells are induced to undergo CSR. YY1 conditional knockout caused differential expression of 1129 genes, with 59 being mitochondrial-related genes. ChIP-seq analyses showed YY1 was directly bound to nearly half of these mitochondrial-related genes. Surprisingly, at the time when YY1 knockout dramatically reduces DNA loop formation and CSR, mitochondrial mass and membrane potential were not significantly impacted, nor was there a significant change in mitochondrial oxygen consumption, extracellular acidification rate, or mitochondrial complex I or IV activities. Our results indicate that YY1 regulates numerous mitochondrial-related genes in splenic B cells, but this does not account for the impact of YY1 on CSR or long-distance DNA loop formation.

Original languageEnglish (US)
Pages (from-to)822-838
Number of pages17
JournalEuropean Journal of Immunology
Volume50
Issue number6
DOIs
StatePublished - Jun 1 2020

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice'. Together they form a unique fingerprint.

  • Cite this

    Nandi, S., Liang, G., Sindhava, V., Angireddy, R., Basu, A., Banerjee, S., Hodawadekar, S., Zhang, Y., Avadhani, N. G., Sen, R., & Atchison, M. L. (2020). YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice. European Journal of Immunology, 50(6), 822-838. https://doi.org/10.1002/eji.201948385