Zebrafish as a model for pancreatic cancer research.

Nelson Shu-Sang Yee, Michael Pack

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Elucidation of basic mechanisms that regulate pancreatic organogenesis may help define molecular pathways involved in the development of exocrine pancreas cancer. The zebrafish has emerged as a powerful model for genetic dissection of the mechanisms underlying vertebrate organogenesis including formation of the pancreas. Unique properties of zebrafish enable genetic and embryological analyses not feasible using other vertebrate model organisms. The optical clarity of the zebrafish embryos allows visual detection of markers for pancreatic morphogenesis and cytodifferentiation by whole mount immunohistochemistry and RNA in situ hybridization. This feature, coupled with the accessibility of the externally fertilized zebrafish embryo and the small size and fecundity of adult zebrafish, facilitates large-scale forward genetic screens using chemical or insertional mutagenesis techniques. Furthermore, these properties allow high throughput studies that target functions of known genes via antisense or enforced expression studies. Together, such studies are predicted to identify novel genes, or known genes essential for pancreas development. Work in zebrafish is predicted to complement research performed using other vertebrate model organisms, and may help identify markers that define early stages of pancreatic tumorigenesis as well as potential targets for therapy.

Original languageEnglish (US)
Pages (from-to)273-298
Number of pages26
JournalMethods in molecular medicine
Volume103
StatePublished - Jan 1 2005

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Zebrafish
Pancreatic Neoplasms
Research
Vertebrates
Organogenesis
Pancreas
Embryonic Structures
Exocrine Pancreas
Insertional Mutagenesis
Genetic Models
Essential Genes
Morphogenesis
Genes
In Situ Hybridization
Fertility
Dissection
Carcinogenesis
Immunohistochemistry
RNA

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

Cite this

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Zebrafish as a model for pancreatic cancer research. / Yee, Nelson Shu-Sang; Pack, Michael.

In: Methods in molecular medicine, Vol. 103, 01.01.2005, p. 273-298.

Research output: Contribution to journalArticle

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