Zinc oxide nanorod integrated microdevice for multiplex virus detection

Xu Yu, Yiqiu Xia, Yi Tang, Wen Long Zhang, Yin Ting Yeh, Huaguang Lu, Si Yang Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Technology development for point-of-care viral pathogen detection is critical for early diagnosis of infectious diseases, and rapid and effective disease intervention. In this paper, we present the development of a zinc oxide nanorod-integrated microdevice for highly sensitive and specific detection of avian influenza virus. This multiplexed immunofluorescence platform takes two advantages of the zinc oxide nanorods. On one hand, the 3D morphology of zinc oxide nanorods efficiently increases the effective surface area for monoclonal antibodies and decreases the diffusion distance between antibody and pathogens. On the other hand, the unique optical property of the translucent randomly ordered zinc oxide nanorod surface enhances fluorescence detection by 30-70%. We demonstrated the detection limit of the H5N2 avian influenza virus could be lowered down to 3.6×103 EID50/mL (EID50: 50% embryo infectious dose), which was about 22 times more sensitive than conventional ELISA assay tested under the same conditions. We further designed the microfluidic biosensor platform to detect multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 avian influenza virus can be released by simply dissolving zinc oxide nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages850-853
Number of pages4
ISBN (Electronic)9781538627310
DOIs
StatePublished - Jul 26 2017
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
Duration: Jun 18 2017Jun 22 2017

Publication series

NameTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan, Province of China
CityKaohsiung
Period6/18/176/22/17

Fingerprint

Zinc Oxide
viruses
Zinc oxide
Nanorods
Viruses
zinc oxides
nanorods
influenza
Pathogens
pathogens
antibodies
Antibodies
platforms
Monoclonal antibodies
Biomarkers
Microfluidics
Biosensors
biomarkers
embryos
infectious diseases

All Science Journal Classification (ASJC) codes

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Yu, X., Xia, Y., Tang, Y., Zhang, W. L., Yeh, Y. T., Lu, H., & Zheng, S. Y. (2017). Zinc oxide nanorod integrated microdevice for multiplex virus detection. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 850-853). [7994182] (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994182
Yu, Xu ; Xia, Yiqiu ; Tang, Yi ; Zhang, Wen Long ; Yeh, Yin Ting ; Lu, Huaguang ; Zheng, Si Yang. / Zinc oxide nanorod integrated microdevice for multiplex virus detection. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 850-853 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).
@inproceedings{1de72cd55a87467cad7615e0fcce56d4,
title = "Zinc oxide nanorod integrated microdevice for multiplex virus detection",
abstract = "Technology development for point-of-care viral pathogen detection is critical for early diagnosis of infectious diseases, and rapid and effective disease intervention. In this paper, we present the development of a zinc oxide nanorod-integrated microdevice for highly sensitive and specific detection of avian influenza virus. This multiplexed immunofluorescence platform takes two advantages of the zinc oxide nanorods. On one hand, the 3D morphology of zinc oxide nanorods efficiently increases the effective surface area for monoclonal antibodies and decreases the diffusion distance between antibody and pathogens. On the other hand, the unique optical property of the translucent randomly ordered zinc oxide nanorod surface enhances fluorescence detection by 30-70{\%}. We demonstrated the detection limit of the H5N2 avian influenza virus could be lowered down to 3.6×103 EID50/mL (EID50: 50{\%} embryo infectious dose), which was about 22 times more sensitive than conventional ELISA assay tested under the same conditions. We further designed the microfluidic biosensor platform to detect multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 avian influenza virus can be released by simply dissolving zinc oxide nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.",
author = "Xu Yu and Yiqiu Xia and Yi Tang and Zhang, {Wen Long} and Yeh, {Yin Ting} and Huaguang Lu and Zheng, {Si Yang}",
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Yu, X, Xia, Y, Tang, Y, Zhang, WL, Yeh, YT, Lu, H & Zheng, SY 2017, Zinc oxide nanorod integrated microdevice for multiplex virus detection. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994182, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 850-853, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, Province of China, 6/18/17. https://doi.org/10.1109/TRANSDUCERS.2017.7994182

Zinc oxide nanorod integrated microdevice for multiplex virus detection. / Yu, Xu; Xia, Yiqiu; Tang, Yi; Zhang, Wen Long; Yeh, Yin Ting; Lu, Huaguang; Zheng, Si Yang.

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 850-853 7994182 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Yeh, Yin Ting

AU - Lu, Huaguang

AU - Zheng, Si Yang

PY - 2017/7/26

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N2 - Technology development for point-of-care viral pathogen detection is critical for early diagnosis of infectious diseases, and rapid and effective disease intervention. In this paper, we present the development of a zinc oxide nanorod-integrated microdevice for highly sensitive and specific detection of avian influenza virus. This multiplexed immunofluorescence platform takes two advantages of the zinc oxide nanorods. On one hand, the 3D morphology of zinc oxide nanorods efficiently increases the effective surface area for monoclonal antibodies and decreases the diffusion distance between antibody and pathogens. On the other hand, the unique optical property of the translucent randomly ordered zinc oxide nanorod surface enhances fluorescence detection by 30-70%. We demonstrated the detection limit of the H5N2 avian influenza virus could be lowered down to 3.6×103 EID50/mL (EID50: 50% embryo infectious dose), which was about 22 times more sensitive than conventional ELISA assay tested under the same conditions. We further designed the microfluidic biosensor platform to detect multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 avian influenza virus can be released by simply dissolving zinc oxide nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.

AB - Technology development for point-of-care viral pathogen detection is critical for early diagnosis of infectious diseases, and rapid and effective disease intervention. In this paper, we present the development of a zinc oxide nanorod-integrated microdevice for highly sensitive and specific detection of avian influenza virus. This multiplexed immunofluorescence platform takes two advantages of the zinc oxide nanorods. On one hand, the 3D morphology of zinc oxide nanorods efficiently increases the effective surface area for monoclonal antibodies and decreases the diffusion distance between antibody and pathogens. On the other hand, the unique optical property of the translucent randomly ordered zinc oxide nanorod surface enhances fluorescence detection by 30-70%. We demonstrated the detection limit of the H5N2 avian influenza virus could be lowered down to 3.6×103 EID50/mL (EID50: 50% embryo infectious dose), which was about 22 times more sensitive than conventional ELISA assay tested under the same conditions. We further designed the microfluidic biosensor platform to detect multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 avian influenza virus can be released by simply dissolving zinc oxide nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.

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Yu X, Xia Y, Tang Y, Zhang WL, Yeh YT, Lu H et al. Zinc oxide nanorod integrated microdevice for multiplex virus detection. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 850-853. 7994182. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/TRANSDUCERS.2017.7994182