@article{ba07391894fd4003841c726a8dedeee9,
title = "ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression",
abstract = "Lan et al. show that the transcription factor ZNF410 has a single direct target gene, the NuRD component CHD4, through which it silences the fetal-type β-globin genes in adult erythroid cells. The exquisite specificity of ZNF410 could be exploited therapeutically for treatment of hemoglobinopathies.",
author = "Xianjiang Lan and Ren Ren and Ruopeng Feng and Ly, {Lana C.} and Yemin Lan and Zhe Zhang and Nicholas Aboreden and Kunhua Qin and Horton, {John R.} and Grevet, {Jeremy D.} and Thiyagaraj Mayuranathan and Osheiza Abdulmalik and Keller, {Cheryl A.} and Belinda Giardine and Hardison, {Ross C.} and Merlin Crossley and Weiss, {Mitchell J.} and Xiaodong Cheng and Junwei Shi and Blobel, {Gerd A.}",
note = "Funding Information: We thank Dr. Shaun Mahony at Penn State University, Dr. Xing Zhang at the University of Texas MD Anderson, and members of the Blobel laboratory for helpful comments and discussions. We acknowledge Drs. Ryo Kurita and Yukio Nakamura for sharing HUDEP-2 cells. We thank the CHOP flow cytometry core for help with cell sorting. We also thank Richard Ashmun, Jonathan Laxton, and Stacie Woolard at St. Jude Children{\textquoteright}s Research Hospital for assistance with flow cytometry; Chandra Savage and Kalin Mayberry for animal husbandry; and Shondra Miller and Shaina Porter for help with gene editing. This work was supported by NIH grants R24DK106766 (to G.A.B. and R.C.H.), R01HL119479 (to G.A.B.), and R35GM134744 (to X.C.); CPRIT RR160029 (to X.C.), Australian National Health and Medical Research Council APP1164920 (to M.C.), a scholarship from the Australian Government Research Training Program (to L.C.L.), the St. Jude -sponsored CRC Consortium , and a fellowship from Cooley's Anemia Foundation (to X.L.). We thank the Di Gaetano family for their generous support. Funding Information: We thank Dr. Shaun Mahony at Penn State University, Dr. Xing Zhang at the University of Texas MD Anderson, and members of the Blobel laboratory for helpful comments and discussions. We acknowledge Drs. Ryo Kurita and Yukio Nakamura for sharing HUDEP-2 cells. We thank the CHOP flow cytometry core for help with cell sorting. We also thank Richard Ashmun, Jonathan Laxton, and Stacie Woolard at St. Jude Children's Research Hospital for assistance with flow cytometry; Chandra Savage and Kalin Mayberry for animal husbandry; and Shondra Miller and Shaina Porter for help with gene editing. This work was supported by NIH grants R24DK106766 (to G.A.B. and R.C.H.), R01HL119479 (to G.A.B.), and R35GM134744 (to X.C.); CPRIT RR160029 (to X.C.), Australian National Health and Medical Research Council APP1164920 (to M.C.), a scholarship from the Australian Government Research Training Program (to L.C.L.), the St. Jude-sponsored CRC Consortium, and a fellowship from Cooley's Anemia Foundation (to X.L.). We thank the Di Gaetano family for their generous support. X.L. J.S. and G.A.B. conceived the study and designed the experiments. X.L. N.A. and O.A. carried out most cell-based experiments. J.D.G. and J.S. performed the CRISPR screen. R.R. performed protein purification, DNA binding assays, and crystallization. R.R. and J.R.H. performed X-ray data collection and structure determination. X.C. organized and designed the scope of the structural study. R.F. and T.M. performed mouse xenotransplantation experiments, and R.F. T.M. and M.J.W. analyzed the data. L.C.L. and M.C. carried out the band retardation experiments. X.L. Y.L. Z.Z. C.A.K. B.G. R.C.H. and K.Q. analyzed ChIP-seq and RNA-seq data. X.L. and G.A.B. wrote the manuscript with input from all authors. X.J. J.S. and G.A.B. are contributors to a patent filed on behalf of the Children's Hospital of Philadelphia. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2021",
month = jan,
day = "21",
doi = "10.1016/j.molcel.2020.11.006",
language = "English (US)",
volume = "81",
pages = "239--254.e8",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "2",
}