ZO-1 tight junction protein expression in rat retinas

S. A. Khin, A. J. Barber, T. W. Gardner

Research output: Contribution to journalArticle

Abstract

Purpose: To determine ZO-1 tight junction protein distribution in control and diabetic rat retinas. Methods: Eighteen of 28 male Sprague-Dawley rats were made diabetic by IV streptozotocin injection, 65 mg/kg. Animals were sacrificed after 7 or 12 weeks of diabetes and the eyes enucleated. ZO-1 indirect immunofluorescence was examined in cryostat sections with a monoclonal antibody. Endothelium was localized with a polyclonal anti-von Willebrand factor antibody, ZO-1 immunoreactivity was estimated by masked evaluation. Results: ZO-1 immunofluorescence was found in vWF-positive microvessels in retina and choroid. ZO-1 immunofluorescence was not detected in the retinal pigment epithelium. ZO-1 immunoreactivity was judged to be reduced in 14/18 diabetic animals versus 3/10 control animals. Conclusions: ZO-1 protein is found in the inner blood-retinal barrier of rats. Experimental diabetes may reduce ZO-1 content and contribute to increased blood-retinal barrier permeability.

Original languageEnglish (US)
Pages (from-to)S971
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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Zonula Occludens-1 Protein
Blood-Retinal Barrier
Retina
Fluorescent Antibody Technique
Choroid
Retinal Pigment Epithelium
von Willebrand Factor
Indirect Fluorescent Antibody Technique
Streptozocin
Microvessels
Endothelium
Sprague Dawley Rats
Permeability
Monoclonal Antibodies
Injections
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

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ZO-1 tight junction protein expression in rat retinas. / Khin, S. A.; Barber, A. J.; Gardner, T. W.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996, p. S971.

Research output: Contribution to journalArticle

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AU - Khin, S. A.

AU - Barber, A. J.

AU - Gardner, T. W.

PY - 1996/2/15

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N2 - Purpose: To determine ZO-1 tight junction protein distribution in control and diabetic rat retinas. Methods: Eighteen of 28 male Sprague-Dawley rats were made diabetic by IV streptozotocin injection, 65 mg/kg. Animals were sacrificed after 7 or 12 weeks of diabetes and the eyes enucleated. ZO-1 indirect immunofluorescence was examined in cryostat sections with a monoclonal antibody. Endothelium was localized with a polyclonal anti-von Willebrand factor antibody, ZO-1 immunoreactivity was estimated by masked evaluation. Results: ZO-1 immunofluorescence was found in vWF-positive microvessels in retina and choroid. ZO-1 immunofluorescence was not detected in the retinal pigment epithelium. ZO-1 immunoreactivity was judged to be reduced in 14/18 diabetic animals versus 3/10 control animals. Conclusions: ZO-1 protein is found in the inner blood-retinal barrier of rats. Experimental diabetes may reduce ZO-1 content and contribute to increased blood-retinal barrier permeability.

AB - Purpose: To determine ZO-1 tight junction protein distribution in control and diabetic rat retinas. Methods: Eighteen of 28 male Sprague-Dawley rats were made diabetic by IV streptozotocin injection, 65 mg/kg. Animals were sacrificed after 7 or 12 weeks of diabetes and the eyes enucleated. ZO-1 indirect immunofluorescence was examined in cryostat sections with a monoclonal antibody. Endothelium was localized with a polyclonal anti-von Willebrand factor antibody, ZO-1 immunoreactivity was estimated by masked evaluation. Results: ZO-1 immunofluorescence was found in vWF-positive microvessels in retina and choroid. ZO-1 immunofluorescence was not detected in the retinal pigment epithelium. ZO-1 immunoreactivity was judged to be reduced in 14/18 diabetic animals versus 3/10 control animals. Conclusions: ZO-1 protein is found in the inner blood-retinal barrier of rats. Experimental diabetes may reduce ZO-1 content and contribute to increased blood-retinal barrier permeability.

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